| 王红梅1，王浩瀚2，岳维云3，张正英1,欧巧明1，陈玉梁1*.抗黄矮病小麦新品系的分子标记检测研究[J].植物保护,2012,38(3):86-89. |
| 抗黄矮病小麦新品系的分子标记检测研究 |
| Detection of wheat lines with resistance to Yellow dwarf virus by molecular markers |
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| DOI： |
| 中文关键词: 小麦黄矮病 分子标记 检测 抗病鉴定 |
| 英文关键词:Barley yellow dwarf virus（BYDV) molecular marker detection identification of disease resistance |
| 基金项目: |
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| 摘要点击次数: 682 |
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| 中文摘要: |
| 本研究对以小麦中间偃麦草异附加系L1和小麦 中间偃麦草部分双二倍体‘无芒中4’为抗源选育出的抗黄矮病小麦新品系进行分子检测和抗病性鉴定。通过应用RAPD、SSR、SCAR 3种分子标记OPF15、Xgwm37、SC W37进行分子检测，并采用人工接种和大田自然感病的方式进行抗黄矮病鉴定，筛选到了‘93646’、‘2003 2’等高抗黄矮病的小麦新品系。分子检测抗黄矮病基因与田间抗病鉴定结果基本一致，应用的3种PCR标记都可以检测出抗病材料，但SCAR标记SC-W37特异性强、稳定性好，可在小麦抗黄矮病育种早代选择过程中发挥重要作用。 |
| 英文摘要: |
| Some BYDV translocation lines were studied by using wheat Thinopyrum intermedium the addition line L1 and a partial amphiploid ‘Zhong 4 awnless’ as the resistance parents. This translocation lines were used to screen molecular markers and identification of disease resistance. A RAPD marker OPF15, SSR marker Xgwm37 and SCAR marker SC W37 were developed for the BYDV resistance. Some new strains with excellent BYDV resistance were selected by identification of disease resistance and molecular marker, including ‘2003 2’ and ‘93646’. The results showed that the BYDV resistant materials could be detected by PCR markers. The SCAR marker SC W37 had high specificity and good reliability, which might play an important role in early generation selection in wheat breeding. |
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