| 摘要: |
| 采用响应面分析法(RSA)研究了提取溶剂、时间和料液比等芹菜黄酮提取条件，比较了提取物粗品的体外抗氧化作用。结果表明，芹菜黄酮提取的最佳工艺条件为：以芹菜干粉为原料，以体积分数40％乙醇为溶剂，料液比1∶15（m∶V）,提取时间2.5 h，提取温度80 ℃。在此最优工艺条件下提取3次，芹菜黄酮提取物粗品得率为5.18%，粗品中黄酮含量为43 g/kg。芹菜黄酮提取物粗品表现出较强的清除羟自由基和1,1－二苯基苦基苯肼（DPPH）自由基的能力，其IC50值分别为10.1和8 mg/L(以黄酮计)，其抗氧化能力略低于槲皮素而强于维生素C。 |
| 关键词: 芹菜 黄酮 提取条件 抗氧化作用 响应面 |
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| 基金项目:广东省自然科学基金重点项目(010133) |
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| Extraction conditions of celery favone and its antioxidative activity |
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| Abstract: |
| The conditions of leaching flavones from celery was studied by response surface analysis and the antioxidative effect of celery flavone extract was compared in vitro.The results showed that the optimal leaching conditions were 40% ethanol,ratio of solid to liquid 1∶15,extracting time 2.5 h,temperature 80 ℃.Under this condition,5.18% extract yield was gained with 4.3% flavone.Celery extract had strong activity of scavenging hydroxyl free radical of Featon reation and DPPH·,whose IC50 values were 10.1 mg/L and 8 mg/L respectively,and its activity was weaker than quercetin while stronger than vitamin C compared with vitamin C and quercetin. |
| Key words: celery flavones extraction conditions response surface antioxidation |
