| 利用紫外线和EMS诱变选育高产枯草芽孢杆菌 |
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| 引用本文： | 卞承荫,黄舒婷,潘大仁,林辉,林冬梅,罗海凌,林兴生,林占熺.利用紫外线和EMS诱变选育高产枯草芽孢杆菌[J].福建农业大学学报,2014(3):312-315. |
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| 作者姓名： | 卞承荫 黄舒婷 潘大仁 林辉 林冬梅 罗海凌 林兴生 林占熺 |
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| 作者单位： | [1]福建农林大学菌草研究户斤、国家菌草工程技术研究中心 [2]福建农林大学作物学院,福建福州350002 |
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| 基金项目： | 科技部国家菌草工程技术研究中心组建资助项目（2011FU125X11）;福建省科技厅重大专项资助项目（2012NZ0002）. |
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| 摘 要： | 以豆豉溶栓酶产生菌株蜡状芽胞杆菌LD-8567为出发菌株，通过紫外线和甲基磺酸乙酯（EMS）的复合诱变选育高产枯草芽孢杆菌．用正交试验法优化EMS的诱变条件，得到最佳诱变条件，即在1mL体系中，EMS用量20μL，诱变时间20min，菌液浓度1×10^8个·mL-1，最终经过初筛和复筛得到了1株产酶活性较高的菌株，对该菌株连续传代发酵培养5代，发现其产酶能力稳定，酶活力保持在16351U·mL-1左右．
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| 关 键 词： | 枯草芽孢杆菌 溶栓酶 紫外诱变 甲基磺酸乙酯（EMS）诱变 诱变育种 |
Breeding of UV and EMS-induced mutant strains of Bacillus subtilis with enhanced thrombolytic enzyme production |
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| BIAN Cheng-yin,HUANG Shu-ting,PAN Da-ren,LIN Hui,LIN Dong-mei,LUO Hai-ling,LIN Sing-sheng,LIN Zhan-.Breeding of UV and EMS-induced mutant strains of Bacillus subtilis with enhanced thrombolytic enzyme production[J].Journal of Fujian Agricultural University,2014(3):312-315. |
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| Authors: | BIAN Cheng-yin HUANG Shu-ting PAN Da-ren LIN Hui LIN Dong-mei LUO Hai-ling LIN Sing-sheng LIN Zhan- |
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| Institution: | xi( 1. Juncao Research Institute, Fujian Agriculture and Forestry University/China National Engineering Research Center of Juncao Technology; 2. College of Corp Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China) |
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| Abstract: | Original strain （Bacillus subtilis LD-8547） was mutated by ultraviolet radiation and EMS induced mutation. Mutagcnesiscondition of EMS was optimized by orthogonal design, that was as follows： EMS was 20 IzL, bacteria liquid concentration was 1 ×10^8 mL, mutagenesis time was 20 win in a system of 1 mL. After two rounds of screening, a strain with high activity was selected.The enzyme activity of mutant was 16351 U·mL-1, which remained stable after five generations. |
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| Keywords: | Bacillus subtills thrembolytle enzyme ultraviolet induced mutation cthylmethylsulfone induced mutation inducedmutation breeding |
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