A modified enzymic browning assay for potato glandular trichomes |
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Authors: | D A Avé N T Eannetta W M Tingey |
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Institution: | 1. Department of Plant Breeding, Cornell University, 14853, Ithaca, New York 2. Department of Entomology, Cornell University, 14853, Ithaca, New York
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Abstract: | Tetralobulate (Type A) glandular trichomes are present on foliage of many wild as well as cultivated species of potatoes. These trichomes confer resistance to insect pests if their density, gland size and biochemical products are similar as those ofSolanum berthaultti Hawkes andS. polyadenium Greenm. Hybrids ofS. tuberosum L. ×S. berthaultii have been screened for resistance by measurement of phenolic oxidation activity in their Type A trichomes by use of an enzymic browning assay. However, variability in the procedure has prompted the development of an improved assay. |
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