桑青枯菌血清型与其它分型的比较研究

 从广东省12个县、市采集了20个桑青枯菌株进行血清学反应、生化反应、寄主范围、烟叶浸润反应、酪氨酸酶活性、致病性、溶源性和菌落特性等试验,并按前人的研究方法划分为相应的血清型、生化型、小种、致病型、溶源型和菌落型等,但这些分型相互间无一定的联系。根据血清学反应的结果,我们把20个桑青枯菌株和花生、番茄、木麻黄和马铃薯等青枯菌株总共划分为8个血清型,桑青枯菌的20个菌株分属于4个血清型。这些桑青枯菌株与花生、番茄和木麻黄等青枯菌株的血清学关系比与马铃薯青枯菌株的关系较为密切。据此我们认为青枯菌种下的分类应以血清型作为主要的分类依据。在血清型下,依次为生化型、小种和致病型等。

STUIES ON THE COMPARISON OF SEROTYPING AND OTHER METHODS OF TYPING OF PSEUDOMONAS SOLANACEARUM FROM MULBERY

Twenty isolates of Pseudomomonas solanacearum from mulbery collected from 12 counties and areas of Guangdong province were classified,according to the conventional methods based on biochemical reaction,host range,pathogenicity,lysogenicity,colony morphology and serological test,as biotype,race,pathotype,lysotype,colony-type and serotype.No any relationship of them to each other was shown in the present studies.With the results of serolpgical tests,20 isolates from mulbery and 4 others from peanut,tomato,potato and beef wood (Casaurina equistifolia) respectively were divided into 8 serotypes;those from mulbery belong to 4 of them.The relationship between the strain of mulbery and that of peanut,tomato,or beef wood was found to be closer than that of potato.It is thus believed that serotyping is supposed to be the primary basis for the classification of the speies of Ps.solauacearum at the sub-specific level;and the subsequent classification of the other types would be in the order as follows:biotypes,races and pathotypes.