| 奶牛隐性乳房炎的主要病原菌的PCR鉴定 |
| |
| 引用本文： | 吴润,郝保青,农向,严丹红.奶牛隐性乳房炎的主要病原菌的PCR鉴定[J].中国牛业科学,2006,33(2):12-14,17. |
| |
| 作者姓名： | 吴润 郝保青 农向 严丹红 |
| |
| 作者单位： | 西南民族大学,生命科学与技术学院,四川,成都,610041 |
| |
| 摘 要： | 基于细菌16SrRNA和（或）23SrRNA序列设计引物，利用PCR的方法鉴定出了奶牛隐性乳房炎的主要病原菌，包括金色葡萄球菌、大肠杆菌和无乳链球菌，避免了繁琐的培养过程，表明PCR技术是细菌鉴定非常有效、特异性高而又快速的方法，对隐乳的诊断很有价值。
|
| 关 键 词： | 隐性乳房炎 病原菌 |
| 文章编号： | 1001-9111（2006）02-0012-03 |
| 收稿时间： | 2005-11-18 |
| 修稿时间： | 2005-11-18 |
Identification of Major Pathogens in Bovine Subclinic Mastitis by PCR |
| |
| WU Run,HAO Bao-qing,NONG Xiang,YAN Dan-hong.Identification of Major Pathogens in Bovine Subclinic Mastitis by PCR[J].China Cattle Science,2006,33(2):12-14,17. |
| |
| Authors: | WU Run HAO Bao-qing NONG Xiang YAN Dan-hong |
| |
| Institution: | College of Life Science and Technology, Southwest Nationalities University, Chengdu, Sichuan 610041, China |
| |
| Abstract: | Absract:Primers were designed based on the DNA sequences of bacterium 16 S rRNA and(or) 23 SrRNA.This article presented a PCR detecting the major pathogens in subclinic mastitis,includingStaphylococcus aoreus,Escherichia coli,Streptococcus agalactiae.The method avoided the redundant cellculturing process,therefore,the result showed that PCR technology is an effective,specific and rapidmolecular tool to identify bacteria,and also a valuable method to diagnose bovine subclinic mastitis. |
| |
| Keywords: | PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
