南宁市肉牛的Y染色体遗传多样性分析

目的]为了探究广西南宁市肉牛的父系遗传背景与遗传组成。方法]利用PCR扩增、限制性酶酶切和生物信息学方法,对南宁屠宰场的73头肉牛Y染色体USP9Y基因的遗传多态性进行分析。结果]发现73头公牛USP9Y基因的PCR产物具有多态性,2头牛显示471 bp带型,71头牛显示552 bp带型。在71个552 bp带型中,有28个可以被SspI酶切成2条带(338 bp和215 bp),表明这28头牛为Y3单倍型组(38.36%),而其余43个不能被SspI酶切,表明这43头牛为Y2单倍型组(58.90%)。仅有2头牛的PCR产物为471 bp,表明这2头牛为Y1单倍型组(2.74%)。屠宰牛群的单倍型多样度为0.5122±0.0309,表明屠宰牛群的Y染色体遗传多样度较高。结论]南宁市屠宰牛群的来源比较复杂,有普通牛(Y1与Y2单倍型组)和瘤牛(Y3单倍型组)2个父系起源。

Y Chromosome Genetic Diversity of Beef Cattle in Nanning City

Objective]To explore the paternal genetic background and composition of beef cattle in Nanning city,Guangxi region.Methods]PCR amplification,restriction enzyme digestion and bioinformatics methods were used to analyze the genetic polymorphism of 73 bulls with USP9Y marker.Results]The results showed that the PCR products of USP9Y gene in 73 bulls showed the amplification polymorphism with 471 bp and 552 bp.The 71 bulls with 552 bp were digested using SspI enzyme,of which 28 bulls showing two bands(338 bp and 215 bp),were Y3 haplogroup(38.36%),while the remaining 43 bulls only showing 552 bp,were Y2 haplogroup(58.90%).The remaining 2 bulls with 471 bp,was Y1 haplogroup(2.74%).The Y chromosome haplotype diversity of the slaughter cattle population was 0.5122±0.0309,suggesting that the slaughter cattle population has relatively high paternal genetic diversity.Conclusion]The study revealed complex composition and two different paternal origins of Bos taurus(Y1 and Y2 haplogroups)and Bos indicus(Y3 haplogroup)of beef cattle in Nanning slaughterhouse.