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| 猪圆环病毒2型厦门株-1的ORF2基因序列分析 | |
| 引用本文： | 孔繁德,徐淑菲,陈琼,黄印尧,陆承平. 猪圆环病毒2型厦门株-1的ORF2基因序列分析[J]. 福建农林大学学报(自然科学版), 2006, 35(2): 177-181 |
| 作者姓名： | 孔繁德 徐淑菲 陈琼 黄印尧 陆承平 |
| 作者单位： | 1. 南京农业大学动物医学院,江苏,南京,210095;厦门出入境检验检疫局,福建,厦门,361012 2. 厦门出入境检验检疫局,福建,厦门,361012 3. 厦门市农产品质量安全检验测试中心,福建,厦门,361009 4. 南京农业大学动物医学院,江苏,南京,210095 |
| 摘 要： | 根据猪圆环病毒2型(PCV2)基因序列,设计合成1对引物,对PCV2厦门株-1(XM-1)的ORF2基因进行PCR扩增.扩增产物经琼脂糖凝胶电泳分析,可清晰看见1条与设计相符的大小为729 bp的特异条带.提取该片段进行测序,并与国内外24株PCV2毒株的ORF2进行比较,发现得到的PCV2 XM-1的ORF2与广西PCV2分离株(AY556475)核苷酸和氨基酸序列同源性均达到100%,与其它PCV2毒株ORF2的同源性分别为91.6%-99.9%和88.9%-100%,表明厦门地区生猪已感染PCV2,PCV2的ORF2基因与其它毒株有所不同. |
| 关 键 词： | 猪圆环病毒 ORF2基因 序列分析 |
| 文章编号： | 1671-5470(2006)02-0177-05 |
| 收稿时间： | 2004-12-20 |
| 修稿时间： | 2005-11-12 |
Sequence analysis of ORF2 gene of porcine circovirus 2(XM-1) |
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| KONG Fan-de,XU Shu-fei,CHEN Qiong,HUANG Yin-yao,LU Cheng-ping. Sequence analysis of ORF2 gene of porcine circovirus 2(XM-1)[J]. Journal of Fujian Agricultural and Forestry University, 2006, 35(2): 177-181 | |
| Authors: | KONG Fan-de XU Shu-fei CHEN Qiong HUANG Yin-yao LU Cheng-ping |
| Affiliation: | 1. College of Veterinary Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; 2. Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen, Fujian 361012, China; 3. Xiamen Agricultural Product Quality and Safety Testing Center, Xiamen, Fujian 361009, China |
| Abstract: | A pair of primers were designed according to the Genbank sequence to amplify ORF2 gene of porcine circovirus Xiamen strain-1(XM-1).The ORF2 gene was obtained by polymerase chain reaction(PCR).The nucleotide and amino-acid sequences of XM-1 ORF2 gene were compared with the counterpart sequences of 24 strains.The nucleotide and animi-acid homologies were 100% with Guangxi strain,91.6%-99.9% or 88.9%-93.7% with 23 other strains.The result showed that some pigs in Xiamen had been infected by PCV2,whose ORF2 gene was different from other isolates. |
| Keywords: | porcine circovirus 2 ORF2 gene sequence analysis |
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