EMS诱变六倍体小麦偃展4110的形态突变体鉴定与分析

 【目的】构建小麦EMS突变体库，为小麦功能基因组学研究准备基础材料。【方法】利用化学诱变剂甲基磺酸乙酯（ethyl methane sulfonate，EMS）诱变处理小麦品种偃展4110种子，将获得的M2代材料进行生物学性状与农艺性状鉴定，部分M3材料播种家系进行验证。【结果】对M2代全生育期田间表型进行观察鉴定，突变群体的表型变异率约为6.6%；获得了幼苗、叶、茎、穗及成熟期等生物学特性与主要农艺性状的变异体和突变体，变异类型丰富，特别是发现了自然突变中少见的变异类型，如株高在10-15 cm左右的特矮变异类型。【结论】本研究所构建的两个偃展4110 EMS突变群体较为理想，可望有效地被用于小麦功能基因组研究和小麦遗传改良中。

Morphological Identification and Analysis of EMS Mutants from Hexaploid Wheat Cultivar Yanzhan 4110

【Objective】 The mutant library is the basic material of functional genomics. In wheat, this kind of mutant library was rarely reported. In this study, two wheat mutant libraries with EMS were constructed for laying a foundation for wheat functional genomics research. 【Method】 EMS was used as chemical mutagen to build the mutant library with early mature spring wheat Yanzhan 4110. Biological characters and agronomic traits were investigated in M2 generation. Some of mutants identified from M2 plants will be validated through M3 generation. 【Result】 During the growth period after sowing the M2 seeds, all the phenotypic changes of plants were observed and recorded. Phenotype mutants such as seedling habits, leaf appearance, plant height, spike appearance and mature period were obtained. Some of them were related to important agronomic traits and biological characters, specifically, some mutants were rarely observed in natural variation, such as extremely dwarf, with plant height at about 10-15cm. The rate of M2 phenotype mutation was 6.6%. 【Conclusion】 Two libraries of Yanzhan 4110 induced with EMS are ideal mutant libraries and they will be efficiently used in wheat functional genomic research and wheat improvement.