| 绵羊肺腺瘤病毒NM株前病毒gag基因的克隆与序列分析 |
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| 引用本文： | 刘淑英,马学恩,李景鹏.绵羊肺腺瘤病毒NM株前病毒gag基因的克隆与序列分析[J].畜牧兽医学报,2005,36(1):54-57. |
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| 作者姓名： | 刘淑英 马学恩 李景鹏 |
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| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018
2. 东北农业大学生命中心基因部,哈尔滨,150030 |
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| 基金项目： | 国家自然科学基金项目(30260083)，内蒙古自治区重点领域项目(ZL9903) |
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| 摘 要： | 参照GenBank中已发表的绵羊肺腺瘤病毒(JSRV)的全基因序列，设计合成3对引物，对JSRVNM株的gag基因分3段进行PCR扩增，经琼脂糖凝胶电泳分析，分别呈3条531、888和949 bp的特异条带，将其分别克隆人pMD-18T载体中，进行序列测定并拼接序列，得到完整的gag基因序列。分析结果表明，与南非代表株(基因序列号NC-001494)的gag基因序列比较，核苷酸同源性为89．0%，推导出的氨基酸同源性为90％。与美国代表株(基因序列号AF105220)的gag基因序列比较，核苷酸同源性为86．3％，氨基酸同源性为87％。
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| 关 键 词： | gag基因 肺腺瘤病 绵羊 氨基酸同源性 序列比较 序列分析 病毒 基因序列 核苷酸 克隆 |
| 文章编号： | 0366-6964(2005)01-0054-04 |
Cloning and Sequencing Analysis gag Gene of Jaagsiekte Sheep Retrovirus Inner Mongolia Strain |
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| LIU Shu-ying,Ma Xue-en,LI Jing-peng.Cloning and Sequencing Analysis gag Gene of Jaagsiekte Sheep Retrovirus Inner Mongolia Strain[J].Acta Veterinaria et Zootechnica Sinica,2005,36(1):54-57. |
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| Authors: | LIU Shu-ying Ma Xue-en LI Jing-peng |
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| Abstract: | In order to amplify gag gene of Jaagsiekte sheep retrovirus Inner Mongolia strain, three pairs of primers were designed according to the GenBank sequence . The fragments of gag gene was obtained by polymerase chain reaction(PCR),then the genes were cloned into pMD-18 T vector and identified by PstI, EcoRI and SalI digestion. The nucleotide and amino acid sequences of NM strain gag gene were compared with the counterpart sequences of South Africa strain(NC-001494) and USA strain (AF105220). The nucleotide and amino acid homology of gag gene were 89.0%,90% and 86.3%,87%, respectively. |
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| Keywords: | Jaagsiekte sheep retrovirus gag gene clone sequencing analysis |
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