Three nonfunctional S-haplotypes in self-compatible tetraploid Chinese cherry (Prunus pseudocerasus L. cv. Taixiaohongying) |
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Authors: | Peng Yu Xuesen Chen Qingwei Meng Yang Zheng Xiang Shen Xiaoliu Chen |
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Affiliation: | (1) Institute of Horticulture Science and Engineering/State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, 271018, Shandong, China;(2) Life Science, Shandong Agricultural University, Taian, 271018, Shandong, China; |
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Abstract: | In tetraploid sour cherry (Prunus cerasus L.), the accumulation of nonfunctional S-haplotypes with disrupted pistil-component (stylar-S) and/or pollen-component (pollen-S) function leads to a genotype-dependent loss of gametophytic self-incompatibility (GSI). Tetraploid Chinese cherry (Prunus pseudocerasus L.) exhibits self-compatibility. In this study, we characterized four S-haplotype-specific F-box protein genes (SFBs) from pollen cDNA of Chinese cherry cv. ‘Taixiaohongying’ with the S-genotype S 1 S 2 S 3 S 4 . Of the four SFBs, three showed typical characteristics of SFBs from other Prunus species and functioned as SFB genes; these were termed SFB a (GQ395341), SFB b (GQ395340), and SFB c (EU267943), respectively. The fourth SFB, termed SFB d (GQ395339), has a 100-bp deletion that resulted in the transcript for SFB d lacking the F-box domain. SFB d lost the pollen-S specific function and functioned as the SFB gene mutant. The results of reverse transcription-PCR analysis indicated that the four SFB genes were transcribed at approximately the same intensity. |
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