Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD |
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Authors: | LI Chen LIU Xuan-xuan ABOUELNASR Hesham MOHAMED HAMED Arisha KOU Meng TANG Wei YAN Hui WANG Xin WANG Xiao-xiao ZHANG Yun-gang LIU Ya-ju GAO Run-fei MA Meng LI Qiang |
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Institution: | 1. Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Sweetpotato Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou 221131, P.R.China;2. School of Life Science, Jiangsu Normal University, Xuzhou 221116, P.R.China;3. Plant Pathology Department, Agriculture and Biology Research Division, National Research Center, Giza 12622, Egypt;4. Department of Horticulture, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt |
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Abstract: | As a critical food crop, sweetpotato (Ipomoea batatas (L.) Lam.) is widely planted all over the world, but it is deeply affected by Sweetpotato Virus Disease (SPVD). The present study utilized short tandem target mimic (STTM) technology to effectively up-regulate the expression of laccase (IbLACs) by successfully inhibiting the expression of miR397. The upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation, including phenylalanine ammonialyase (PAL), 4-coumarate-CoAligase (4CL), hydroxycinnamoyl CoA:shikimatetransferase (HTC), caffeicacid O-methyltransferase (COMT), and cinnamyl alcohol dehydrogenase (CAD). Meanwhile, the activities of PAL and LAC increased significantly, finally leading to increased lignin content. Lignin deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants, reduced the accumulation of SPVD transmitted by Bemisia tabaci (Gennadius), and promoted healthy sweetpotato growth. The results provide new insights for disease resistance breeding and green production of sweetpotato. |
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Keywords: | sweetpotato miR397 STTM SPVD lignin content |
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