整合微生物组菌剂对番茄种子萌发和幼苗生长的影响及其对青枯病的防治效果

整合微生物组菌剂是利用微生物发酵床作为发酵槽，通过二次好氧发酵生产含有多个功能微生物组的一种新型土壤连作障碍修复剂。本研究采用室内培养皿法，研究整合微生物组菌剂不同浸提液体浓度对番茄种子萌发的影响，结果表明，整合微生物组菌剂浸提液的100倍稀释液处理的番茄种子发芽指数（36.75）和种子活力指数（106.07）均显著高于无菌水对照（发芽指数33.98，种子活力指数65.26）；整合微生物组菌剂浸提液的稀释倍数低于50倍会抑制番茄种子萌发，其发芽率、发芽指数和种子活力指数均显著低于无菌水对照。通过盆栽试验，研究不同添加量整合微生物组菌剂对番茄植株生长及青枯病害的防治效果，结果表明，低浓度整合微生物组菌剂（添加量为小于60%）会促进番茄植株生长，而高浓度整合生物组菌剂（添加量大于80%）抑制番茄植株生长；添加量为40%，其促长效果最好，植株的株高、茎粗和根长分别比对照增加13.77%、26.00%和79.51%，其根系活力31.15 μg/（g·h），也显著高于对照组24.72 μg/（g·h）；整合微生物组菌剂对番茄青枯病的防治效果随添加量增大而增加，添加量大等于40%，其对番茄青枯病的防治效果均大于65%，添加量为100%的防治效果最好，为77.37%。综合促长效果、青枯病的防治效果及成本，建议整合微生物组菌剂使用剂量为添加量40%。

Effects of an Integrated Microbiome Agent on Tomato Seed Germination and Plant Growth,and Its Control Efficiency against Bacterial Wilt

The integrated microbiome agent is a new type of soil amendment for continuous cropping obstacle, which was manufactured by using the microbial fermentation bed of pig raising as a fermentation tank. In this study, the effect of the integrated microbiome agent extract with different dilution ratio on tomato seed germination was analyzed by using indoor Petri dish method. Compared to sterile water control, the extract with 100 dilution ratio had significant higher of seed germination index (33.98) and vigor index (65.26), while the extract with less than 50 dilution ratio significantly inhibited seed germination percentage, germination index and vigor index (P<0.05). Meanwhile, the effect of the integrated microbiome agent with different dosage on tomato plant growth and its control efficiency against bacterial wilt were also tested. The results showed that the integrated microbiome agent with low dosage (<60%) could improve plant growth, while that with high dosage (>80%) could inhibit plant growth. Compared to sterile water control, the treatment of adding 40% of integrated microbiome agent could increase plant height, stem diameter and root length with 13.77%, 26.00% and 79.51%, respectively. The root activity for adding 40% of integrated microbiome agent was 31.15μg/g/h, which was significant higher than that for sterile water control (24.72 μg/g/h) (P<0.05). The result of biological test also showed that the control efficiency of the integrated microbiome agent addition was increased by the increasing dosage. The control efficiency was above 65% for the dosage more than 40%, and the dosage of 100% had the highest control efficiency of 77.37%. Based on the assessments of grow promotion, control efficiency and cost, the dosage of 40% was suggested for using the integrated microbiome agent.