| 以栉孔扇贝闭壳肌为例的几种DNA提取方法的比较研究 |
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| 引用本文： | 王敏强 张晓雯 王小涛 孙力 龚雪琴. 以栉孔扇贝闭壳肌为例的几种DNA提取方法的比较研究[J]. 齐鲁渔业, 2004, 21(9): 6-8 |
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| 作者姓名： | 王敏强 张晓雯 王小涛 孙力 龚雪琴 |
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| 作者单位： | 烟台大学化学生物理工学院 264005(王敏强，张晓雯，王小涛，孙力)，烟台大学化学生物理工学院 264005(龚雪琴) |
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| 摘 要： | 以栉孔扇贝(Chlamys farreri)闭壳肌为试验材料，采用浓盐法、酚抽提法、LiCl法及改进的浓盐法和酚抽提法等提取其DNA，通过紫外吸收值测定及琼脂糖凝胶电泳法对提取的DNA质量进行分析比较。结果表明，这5种方法都能提到DNA，但改进的浓盐法和酚抽提法可将其中的蛋白质及RNA较为有效地除去，得到比较纯净的DNA。
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| 关 键 词： | 栉孔扇贝 闭壳肌 DNA 提取方法 浓盐法 酚抽提法 琼脂糖凝胶电泳 |
Methods of Extraction DNA from Closing Shell Muscle of Chlamys farreri |
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| Wang Minqiang et al. Methods of Extraction DNA from Closing Shell Muscle of Chlamys farreri[J]. Shandong Fisheries, 2004, 21(9): 6-8 |
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| Authors: | Wang Minqiang et al |
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| Affiliation: | Wang Minqiang et al |
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| Abstract: | Five methods were used for extracting DNA from Chlamys farreri closing shell muscle,they are dense salt method,phenol method and LiCl method. The other two are improved dense salt and phenol method.Ultraviolet absorption and Agarose Gel Electrophoresis are used to compared the quality of the DNA. The results showed that all of these five methods can extract the Chlamys farreri DNA,but with improved dense salt and phenol method,purer DNA can be abtained by getting rid of the protein and RNA. |
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| Keywords: | Chlamys farreri DNA extraction ultraviolet absorption agarose gel electrophoresis |
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