Establishment of cell suspension line of <Emphasis Type="Italic">Populus tomentosa</Emphasis> Carr. |
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Authors: | Na Yao Zhi-yi Zhang Xin-min An Kai Yang |
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Institution: | (1) Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, 100083, P. R. China;(2) Department of Biotechnology, Beijing Agricultural College, Beijing, 102206, P. R. China |
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Abstract: | Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus
induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid
medium supplemented with 1.0 mg·L−1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented
with 1.5 mg·L−1 2,4-D. The best subculture medium was MS + 0.8 mg·L−1 2,4-D + 30 g·L−1 sucrose with a subculture cycle of seven days. |
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Keywords: | Populus tomentosa callus cell suspension line |
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