鲤慢性喹乙醇中毒的病理学和组织残留

试验以含10、25、50、100、150和200mg·kg-1喹乙醇的饲料对鲤进行了慢性毒性试验。经90d的试验,各组的发病率分别为17.5%、27.5%、35.0%、42.5%、47.5%和55.0%,死亡率分别为5.0%、10.0%、12.5%、17.5%、20%和30.0%。中毒鱼表现为特征性的"应激性出血综合征",且Hb含量和RBC数量降低,血清AST、ALT活性升高。病理组织学表现为鳃小片水肿,上皮细胞增生、变性、坏死和脱落。心肌纤维、肾小管上皮细胞和肾上腺细胞空泡变性;肝细胞脂肪变性或水泡变性及溶解坏死;肠道发生卡他性肠炎;超微结构上,心肌纤维、肝细胞和肾小管上皮细胞的线粒体肿胀、嵴断裂、溶解,粗面内质网扩张,核糖体脱颗粒;肝细胞内脂滴明显增多;肠上皮微绒毛脱落、崩解和肠上皮细胞大量坏死脱落。中毒鱼肝、肾和肌肉组织内喹乙醇含量与对照组之间有显著性差异。

Studies on pathology and tissue concentration of the chronic olaquindox poisoning in Cyprinus carpio

The pathology and tissue concentrat ion of the chronic olaquindox poisoning in common carp, Cyprinus carpio , were studied. The test was conducted for 90 days, with doses of 10, 25, 50, 100, 150 and 200mg#kg- 1 feed. The morbidity and mortality were 17. 5% , 27. 5%, 35. 0% , 42. 5%, 47. 5% , 55. 0% and 5. 0% , 10. 0%, 12. 5% , 17. 5% , 20. 0%, 30. 0% respectively. The clinic symptom of the poisoned fish was characterized by / the stress hemorrhage syndrome0. The lesions of poisoned cases showed hyperplasia, degeneration , necrosis and desquamation in the gill lamella epithelia, fatty or vacuolar degeneration and necrosis in the liver cells, the catarrhal enteritis, vacuolar degeneration in renal tubular epithelial cells, cardiac muscle cells and the adrenal gland cells. Ultrastructurally, the mitochondria of the cardiac muscle cells, the liver cells and renal tubular epithelial cells were swollen with disintegration and lysis of caritas. The fat drops increased in liver cells. The intestinal epithelium microvillis collapsed and desquamated at the first stage of the test , and then many intestinal epitheliums necrosed and desquamated. Hematologically , the Hb concentration and the RBC numbers dropped; the serum AST and ALT activity were raised. The olaquindox concentration in the liver, kidney and muscle of the test groups was signif icantly different from the control group.