| 中国林蛙Sox基因的PCR扩增 |
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| 引用本文： | 白秀娟,李剑虹,顾志刚. 中国林蛙Sox基因的PCR扩增[J]. 经济动物学报, 2001, 5(3): 37-39 |
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| 作者姓名： | 白秀娟 李剑虹 顾志刚 |
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| 作者单位： | 1. 吉林农业大学中药材学院,长春,130118
2. 东北农业大学动物科技学院,黑龙江,哈尔滨,150030 |
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| 摘 要： | 以特异扩增人SRY基因HMG -box保守区的一对引物 ,扩增了中国林蛙Sox基因(SRY -boxgene)。结果表明 ,雄性中国林蛙扩增出了两条带 ,分子长分别为 2 5 0bp和 60 0bp ,而雌性个体中未见扩增带 ,显示了中国林蛙Sox基因在雌雄性别间有差异。本研究为探讨中国林蛙的性别决定机制及性别控制的早期鉴定提供了分子依据
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| 关 键 词： | 中国林蛙 Sox基因 PCR扩增 |
| 文章编号： | 1007-7448(2001)03-0037-03 |
PCR Amplification of Sox Gene of Rona temperaria chensinensis |
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| BAI Xiu juan ,LI Jian hong ,GU Zhi gang. PCR Amplification of Sox Gene of Rona temperaria chensinensis[J]. Journal of Economic Animal, 2001, 5(3): 37-39 |
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| Authors: | BAI Xiu juan LI Jian hong GU Zhi gang |
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| Affiliation: | BAI Xiu juan 1,LI Jian hong 2,GU Zhi gang 2 |
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| Abstract: | Sox gene of Rona temperaria chensinensis was amplified with PCR with a primer specifically used for amplifying HMG box conservative region of human SRY gene. Two bands (250bp and 600bp) was found in male frog, while no bands were found in female frog. The results indicated that there is difference in Sox gene in male and female frogs. |
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| Keywords: | Rona temperaria chensinensis Sox gene PCR |
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