| 秋水仙素诱导文旦柚2n配子获得自交三倍体 |
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| 引用本文： | 杨晓伶,程舟,李珊,北岛 宣,长谷川耕二郎.秋水仙素诱导文旦柚2n配子获得自交三倍体[J].园艺学报,2006,33(5):1045-1047. |
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| 作者姓名： | 杨晓伶 程舟 李珊 北岛 宣 长谷川耕二郎 |
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| 作者单位： | (同济大学生命科学与技术学院, 上海200092; 日本京都大学附属农场, 大阪56920096, 日本; 日本高知大学农学部, 高知78328502, 日本) |
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| 摘 要： | 以‘土佐文旦’柚成树为材料, 探讨了秋水仙素诱导2n配子的方法, 研究了秋水仙素浓度和处理时期对获得三倍体的影响。结果表明: 开花前1周用0.25%的秋水仙素处理花蕾, 可诱导2n配子自交亲和。经对发芽存活的自交后代植株的叶片细胞核DNA量检测和细胞学鉴定, 确定25%的自交后代植株为自交三倍体。通过秋水仙素诱导文旦2n配子是获得自交三倍体的有效途径。
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| 关 键 词： | 柚 秋水仙素 2n配子 三倍体 |
| 文章编号： | 0513-353X（2006）05-1045-03 |
| 收稿时间： | 2005-11-06 |
| 修稿时间： | 2005-11-062006-02-23 |
Self-hybridized Triploids Derived from 2n Gametes Induced by Colchicine in Pummelo |
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| Yang Xiaoling,Cheng Zhou,Li Shan,Kitajima Akira,Hasegawa Kojiro.Self-hybridized Triploids Derived from 2n Gametes Induced by Colchicine in Pummelo[J].Acta Horticulturae Sinica,2006,33(5):1045-1047. |
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| Authors: | Yang Xiaoling Cheng Zhou Li Shan Kitajima Akira Hasegawa Kojiro |
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| Institution: | (School of Life Science and Technology, Tongji University, Shanghai 200092, China; Experimental Farm, Kyoto University,Osaka 56920096, Japan; Faculty of Agriculture, Kochi University, Kochi 78328502, Japan) |
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| Abstract: | Tosa-Buntan pummeloCitrus grandis(L.)Osb. was used to establish a method of 2n gametes induced by colchicine. The effects of colchicine concentration and its treatment period on the derived self-hybridized triploids were also analyzed. The results showed that 2n gametes with self-compatibility could be induced by treating the bud with 0.25% colchicine at 1 week before flowering. 25% of the alive F_1 plants were considered as the self-hybridized triploids after the measurement of leave cellular nucleus DNA content and cytological identification. Therefore, it is the effective method to obtain the self-hybridized triploids derived from 2n gametes induced by colchicines in pummelo. |
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| Keywords: | Pummelo Colchicine 2n gamete Triploid |
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