猪肌生成抑制素(MSTN)基因cDNA克隆及序列分析

利用GenBank中猪肌生成抑制素(MSTN)编码序列设计引物,以湖北白猪背最长肌肌细胞总RNA为模板,利用RT-PCR技术,扩增出MSTN基因cDNA片段。该片段全长1277bp,包含猪MSTN基因的全部编码序列。将所得片段与pUCm-T载体连接,转化到DH5α大肠杆菌中,成功地筛选到阳性克隆,其质粒测序结果与文献报道的一致。

Cloning and Sequencing of Hubei White Swine Myostatin Gene cDNA

Total RNA of muscle cell was prepared from a pig breed Hubei white swine and was used to amplify myostatin(MSTN) gene cDNA by RT-PCR.The resulted cDNA fragment with the expected size was cloned into vector pUCm-T and identified by digestion with restrictive enzyme before being sequenced.The result showed that the cloned MSTN gene was 1277 bp in total length and comprised the complete coding sequence which was 99.5% homogenous to the reported swine MSTN genes with 3 nucleotides replacement and only one amino acid difference.