Effects of pentachlorophenol on asymbiotic nitrogen fixation in soil |
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Authors: | T -Y Tam J T Trevors |
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Institution: | 1. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada
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Abstract: | The effect of 50, 100, 150, and 400 μg sodium pentachlorophenate (Na-PCP) per gram soil was studied in nonsterile soil incubated under aerobic and anaerobic conditions, and in sterilized soil inoculated withAzotobacter sp. isolated from the soil. N2 fixation was determined by acetylene reduction. Pentachlorophenate at a concentration of 50 μg g?1 had an inhibitory effect in nonsterile soil incubated aerobically while strong inhibition of dinitrogen fixation in nonsterile soil occurred in the presence of 100 μg g?1 and above. The EC50 values for the inhibition of nitrogenase activity in nonsterile soil incubated aerobically and anaerobically and in sterilized soil inoculated withAzotobacter sp. suspensions were 49.8±1.4 μg Na-PCP g?1, 186.8±2.8 μg Na-PCP g?1, and 660.8±29.3 μg Na-PCP g?1, respectively. |
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