| 康乃馨脱除斑驳病毒组织培养技术研究 |
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| 引用本文： | 裴智能,吴光金. 康乃馨脱除斑驳病毒组织培养技术研究[J]. 湖北林业科技, 2010, 0(4): 31-33,49 |
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| 作者姓名： | 裴智能 吴光金 |
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| 作者单位： | 湖北省当阳市森林病虫害防治检疫站,当阳,444100;中南林业科技大学,长沙,421000 |
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| 摘 要： | 作者对康乃馨进行脱除斑驳病毒培育无毒苗的技术研究。试验表明:用0.3mm茎尖接种,在MS+BA0.7mg/L+NAA0.1～0.2mg/L培养基上愈伤组织诱导率为90.0%;在MS+BA0.8mg/L+NAA0.2mg/L培养基上愈伤组织分化出芽率为74.0%,平均每块愈伤组织有芽5.9个。在MS+BA2.0mg/L+NAA0.3mg/L和MS+BA2.0mg/L+NAA0.5mg/L培养基上增殖倍数6.5～7.4;在1/2MS+NAA0.01mg/L+IBA0.05mg/L上诱导生根率为90.0%;降低培养温度、增加光照强度及培养基中加入20万单位青霉素可减轻玻璃苗现象。经汁液传染试验检测,组培苗脱毒率为65.0%。
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| 关 键 词： | 康乃馨 组织培养 脱毒苗 斑驳病毒 |
Study on Tissue Culture of Dianthus fragrans Fisch Removal Mottle Virus |
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| Pei Zhineng,Wu Guangjin. Study on Tissue Culture of Dianthus fragrans Fisch Removal Mottle Virus[J]. Hubei Forestry Science and Technology, 2010, 0(4): 31-33,49 |
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| Authors: | Pei Zhineng Wu Guangjin |
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| Affiliation: | (2)(1.Station for Forest Pest and Disease Control and Quarantine in Dangyang City of Hubei Dayang 44100 2.Central South University of Forestry and Technology Changsha 421000) |
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| Abstract: | the result showed that a length of 0.3 mm stem tips was inoculated.Callus induction rate is 90.0% in culture medium of MS＋BA0.7mg/L＋NAA0.1-0.2mg/L,Callus germination rate is 74.0%in MS＋ BA0.8mg/L＋NAA0.2 mg/L,and there are 5.9 buds per callus,propagation multiple is 6.5～7.4 of culture medium in MS＋BA2.0mg/L＋NAA0.5mg/L,induction rooting rate is 90%in1/2MS＋NAA0.01 mg/L＋ IBA0.05 mg/L,Reduce incubation temperature,increasing light intensity and medium supplemented with 200 000 units of penicillin can reduce vitrification phenomenon.Tissue culture seedling removal rate is 65.0% by the sap transmission test. |
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| Keywords: | Dianthus fragans Fisch tissue culture virus-free seeding mottle virus |
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