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蒽酮-硫酸法测定芝芪菌质中的多糖含量
引用本文:阮鸣.蒽酮-硫酸法测定芝芪菌质中的多糖含量[J].安徽农业科学,2008,36(26).
作者姓名:阮鸣
作者单位:南京晓庄学院生命科学系药用菌物研究所,江苏南京,211171
基金项目:南京晓庄学院校科研和教改项目
摘    要:用蒽酮—硫酸法测定芝芪菌质中的多糖含量。研究确定了蒽酮-硫酸法测定芝芪菌质中多糖含量的最佳检测条件,并测定了3批芝芪菌质的多糖含量。研究得出,当加入6.0 ml、0.050%的硫酸蒽酮溶液,在水浴中加热20 min,冷却20 min时,芝芪菌质中多糖含量测定条件最优;3批芝芪菌质多糖的平均含量为0.991 3%。

关 键 词:芝芪菌质  多糖  蒽酮-硫酸法  含量测定

Determination of Polysaccharide Content in Zhiqi Fungal Substance by Anthrone-sulfuric Acid Method
RUAN Ming.Determination of Polysaccharide Content in Zhiqi Fungal Substance by Anthrone-sulfuric Acid Method[J].Journal of Anhui Agricultural Sciences,2008,36(26).
Authors:RUAN Ming
Abstract:Anthrone-sulfuric acid method was used to determine the content of polysaccharide in Zhiqi fungal substance.The optimal determination conditions for polysaccharide content in Zhiqi fungal substance by anthrone-sulfuric acid method were studied.And the polysaccharide contents in three batches of Zhiqi fungal substance were detected.The determination condition was the optimal when adding 6 ml of 0.05% anthrone-sulfuric acid,heating for 20 min and cooling for 20 min.The average content of polysaccharide in three batches of Zhiqi fungal substance was 0.991 3%.
Keywords:Zhiqi fungal substance  Polysaccharide  Anthrone-sulfuric acid method  Content determination
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