| 陕西省地方小麦品种多酚氧化酶基因等位变异检测及分布 |
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| 引用本文： | 邓彪,屈国胜,王茹,张晓科,孙道杰,杨松杰. 陕西省地方小麦品种多酚氧化酶基因等位变异检测及分布[J]. 分子植物育种, 2009, 7(3). DOI: 10.3969/mpb.007.000478 |
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| 作者姓名： | 邓彪 屈国胜 王茹 张晓科 孙道杰 杨松杰 |
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| 作者单位： | 1. 安康学院农学与生命科学院,安康,725000
2. 西北农林科技大学农学院,杨凌,712100 |
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| 基金项目： | 陕西教育厅项目，安康学院人才启动专项经费项目 |
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| 摘 要： | 小麦籽粒中多酚氧化酶(polyphenol oxidase,PPO)基因活性与面条等面制品颜色褐变密切相关,降低籽粒PPO活性是小麦品质改良的重要目标.利用PPO基因的功能标记PPO18和PPO29,对115份陕西省地方冬小麦品种(系)进行2A和2D染色体上PPO等位基因印Ppo-A 1a、Ppo-A1b和Ppo-D1b检测,并分析了品种等位变异的分布特点.结果显示:PPO18在等位基因Ppo-A1a(高PPO)和Ppo-A1b(低PPO)中分别扩增685 bp和876 bp的片段,PP029在Ppo-D1b(高PPO)等位基因中扩增490bp的片段.115份品种(系)中Ppo-A1a、Ppo-A1b6和Ppo-D1b等位基因的频率分别为37.39%、60.00%和45.22%.在陕西省所培育和引进的冬小麦品种(系)中,地方当家品种中最低PPO活性基因分布较多,而引进的冬小麦品种中高PPO活性基因分布较多.
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| 关 键 词： | 小麦 PPO基因 分子标记辅助育种(MAS) |
Molecular Identification and Distribution of the Polyphenol Oxidase Genes in Shaanxi Winter Wheat Cultivars |
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| Deng Biao,Qu Guosheng,Wang Ru,Zhang Xiaoke,Sun Daojie,Yang Songjie. Molecular Identification and Distribution of the Polyphenol Oxidase Genes in Shaanxi Winter Wheat Cultivars[J]. Molecular Plant Breeding, 2009, 7(3). DOI: 10.3969/mpb.007.000478 |
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| Authors: | Deng Biao Qu Guosheng Wang Ru Zhang Xiaoke Sun Daojie Yang Songjie |
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| Affiliation: | Deng Biao 1 Qu Guosheng 1 Wang Ru 1 Zhang Xiaoke 2 Sun Daojie 2 Yang Songjie 1 1 College of Agriculture , Life Sciences,Ankang University,Ankang,725000,2 College of Agronomy,Northwest Agriculture , Forestry University,Yangling,712100 |
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| Abstract: | The aims of this study were to characterize the allelic distribution of PPO genes in Shaanxi winter wheat cultivars with STS molecular markers and analyze the association between PPO activity and different PPO alleles for the improvement of PPO activity in marker-assisted selection (MAS). A total of 115 Shaanxi wheat cultivars and advanced lines were tested with the functional markers PPO18 and PPO29 for the identification of three allelic variants Ppo-A1a, Ppo-A1b and Ppo-D1b on chromosomes 2A and 2D. The ... |
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