| 百合离体抗盐变异体的筛选及生理特性研究 |
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| 引用本文： | 刘艳妮,王飞. 百合离体抗盐变异体的筛选及生理特性研究[J]. 西北林学院学报, 2010, 25(2): 70-75 |
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| 作者姓名： | 刘艳妮 王飞 |
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| 作者单位： | 西北农林科技大学园艺学院; |
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| 基金项目： | 陕西省科技攻关项目"花卉新品种引进与示范" |
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| 摘 要： | 用甲基磺酸乙酯(EMS)对百合离体叶片进行了处理。结果表明,随着处理浓度和处理时间的增加,叶片的分化率和存活率均下降,并筛选出其半致死浓度(0.6%)和处理时间(3 h)。利用筛选的EMS浓度和处理时间对离体叶片进行处理,在1%盐浓度胁迫下进行抗盐性突变体筛选,得到抗盐性诱变植株。经鉴定,其超氧化物歧化酶(SOD)、过氧化物酶(POD)活性增强,丙二醛(MDA)含量减少,细胞膜透性减小,可溶性蛋白含量增加,表明筛选的百合突变体比对照具有更强的抗盐性。
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| 关 键 词： | 亚洲百合(Liliumlanci folium) EMS 诱变 耐盐性鉴定 |
Selection of Salt-resistant Mutant of Lily in Vitro and Study on Its Physiological Characteristics |
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| LIU Yan-ni,WANG Fei. Selection of Salt-resistant Mutant of Lily in Vitro and Study on Its Physiological Characteristics[J]. Journal of Northwest Forestry University, 2010, 25(2): 70-75 |
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| Authors: | LIU Yan-ni WANG Fei |
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| Affiliation: | LIU Yan-ni,WANG Fei(College of Horiicultare,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | The leaves of lily in vitro were treated with EMS.With the increase of processing time and concentration,the differentiation and survival rates of lily leaves decreased.The semi-lethal concentration of EMS(0.6 %) and processing time(3 h)were selected.Lily leaves treated with the selected EMS concentration and processing time were selected at 1% salinity stress.Mutant with stronger resistence was gained.The result of the identification showed the selected mutant has more superoxide dismutase(SOD),peroxidase(... |
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| Keywords: | lancifolium) EMS lily EMS induced mutation salt tolerance identification |
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