| EGF和不同血清对牛卵母细胞成熟及核移植胚体外发育的影响 |
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| 引用本文： | 王亚刚,隋丽娜,彭新荣. EGF和不同血清对牛卵母细胞成熟及核移植胚体外发育的影响[J]. 西北农林科技大学学报(自然科学版), 2007, 35(10): 25-28 |
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| 作者姓名： | 王亚刚 隋丽娜 彭新荣 |
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| 作者单位： | 1. 西北农林科技大学,生物工程研究所,陕西,杨凌,712100
2. 西北农林科技大学,生物工程研究所,陕西,杨凌,712100;新疆石河子大学动物科技学院,新疆,石河子,832003 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 分离牛卵母细胞,分别用添加表皮生长因子(EGF)和发情牛血清(发情当天血清(OCS(0 d))和发情第7天血清(OCS(7 d)))的培养液进行成熟培养后去核,构建牛体细胞核移植胚并进行体外培养,研究EGF和OCS对卵母细胞成熟及核移植胚体外发育的影响。结果表明EGF添加组和OCS(0 d)添加组卵细胞的成熟率及核移植胚的卵裂率和囊胚率分别为74.5%,84.6%,25.9%和83.9%,78.8%,31.3%。在牛卵母细胞成熟液中添加EGF、OCS(0 d)更有利于牛核移植胚胎的发育。
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| 关 键 词： | 表皮生长因子 发情牛血清 胎牛血清 核移植胚 |
| 文章编号： | 1671-9387（2007）10-0025-04 |
| 收稿时间： | 2006-09-04 |
| 修稿时间： | 2006-09-04 |
Effect of EGF and different serum on the maturation of bovine oocytes and the development of bovinesomatic nuclear transfer embryos in vitro |
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| WANAG Ya-gang,SUI Li-na,PENG Xin-rong,SAU Wu-jiafu,GUO Zhi-lin,LI Xiang-chen,WANG Yong-sheng,ZHANG Yong. Effect of EGF and different serum on the maturation of bovine oocytes and the development of bovinesomatic nuclear transfer embryos in vitro[J]. Journal of Northwest A&F University（Natural Science Edition）, 2007, 35(10): 25-28 |
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| Authors: | WANAG Ya-gang SUI Li-na PENG Xin-rong SAU Wu-jiafu GUO Zhi-lin LI Xiang-chen WANG Yong-sheng ZHANG Yong |
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| Affiliation: | 1 (1 Institution of Bio-Engineering,Northwest A & F University,Yangling,Shaanxi 712100,China;2 College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832003,China) |
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| Abstract: | Oocytes were isolated from bovine ovaries,enucleated after maturation in maturation medium with EGF or OCS(0 d and 7 d),respectively.Nuclear transfer embryos were constructed and cultured in vitro,and the influence of EGF and OCS on oocyte maturation was also studied.The results showed that the rates of maturation,cleavage and blastoaysts on group adding EGF and group adding OCS(0 d) were 74.5%,84.6%,25.9% and 83.4%,78.7%,31.3%,respectively.The results indicate that the nuclear transfer embryos' development is better in the oocytes maturation medium with EGF or OSC(0d). |
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| Keywords: | EGF OCS FBS nuclear transfer embryos |
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