| 超临界CO_2杀灭大肠杆菌过程中菌体蛋白的变化 |
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| 引用本文： | 饶伟丽,张德权,李淑荣,李春红,朱捷.超临界CO_2杀灭大肠杆菌过程中菌体蛋白的变化[J].核农学报,2009,23(3). |
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| 作者姓名： | 饶伟丽 张德权 李淑荣 李春红 朱捷 |
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| 作者单位： | 中国农业科学院农产品加工研究所,农业部农产品加工与质量控制重点开放实验室,北京,100193 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，中国农业科学院院长基金 |
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| 摘 要： | 通过SDS-聚丙烯酰胺凝胶电泳,结合超临界CO2对大肠杆菌的杀菌曲线,研究超临界压力、温度和处理时间对大肠杆菌菌体蛋白的影响.结果表明:超临界CO2在杀灭大肠杆菌过程中,可显著降低菌体碱溶性(pH8.0)蛋白的溶解性,但对菌体总蛋白含量和种类没有影响;37℃下超临界CO2处理大肠杆菌30min,压力为10MPa时,大肠杆菌存活率为2.8%,菌体碱溶性蛋白的溶解性显著降低,当压力增加到50MPa时,大肠杆菌存活率和碱溶性蛋白溶解性变化不明显;超临界温度和处理时间对菌体碱溶性蛋白溶解性的影响与大肠杆菌在超临界CO2中的存活趋势基本一致,随处理时间的延长和温度的增加大肠杆菌存活率逐渐降低到0,碱溶性蛋白溶解性逐渐降低,直至部分蛋白带消失,但菌体总蛋白含量和种类并没有明显变化;升高温度比增加压力更能显著地导致菌体碱溶性蛋白变性,溶解性下降.
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| 关 键 词： | 超临界CO2 大肠杆菌 杀灭作用 碱溶性蛋白 总蛋白 溶解性 |
THE PROTEIN CHANGE DURING THE INACTIVATION OF Escherichia coli BY SUPERCRITICAL CO_2 |
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| RAO Wei-li,ZHANG De-quan,LI Shu-rong,LI Chun-hong,ZHU Jie.THE PROTEIN CHANGE DURING THE INACTIVATION OF Escherichia coli BY SUPERCRITICAL CO_2[J].Acta Agriculturae Nucleatae Sinica,2009,23(3). |
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| Authors: | RAO Wei-li ZHANG De-quan LI Shu-rong LI Chun-hong ZHU Jie |
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| Institution: | Institute of Agro-Food Science and Technology;Chinese Academy of Agricultural Sciences;Key Laboratory of Agricultural Product Processing and Quality Control;Ministry of Agriculture;Beijing 100193 |
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| Abstract: | The influences of supercritical CO_2 on the total protein and alkali-soluble protein of E.coli was studied by SDS-PAGE analysis and the inactivation curve of E.coli by supercritical CO_2.The results showed that the solubility of E.coli alkali-soluble protein(pH8.0) significantly decreased when E.coli was inactivated by supercritical CO_2,but there was no change of the species and content of total protein of E.coli after supercritical CO_2 treatment.The solubility of alkali-soluble protein significantly decr... |
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| Keywords: | supercritical CO_2 E coli inactivation alkali-soluble protein total protein solubility |
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