DNA-RAPDs detect genetic variation and paternity in Malus |
| |
Authors: | T Harada K Matsukawa T Sato R Ishikawa M Niizeki K Saito |
| |
Institution: | (1) Division of Bioresources, Faculty of Agriculture, Hirosaki University, 036 Hirosaki, Japan;(2) Aomori Agricultural Experimental Station, 036 Kuroishi, Japan;(3) Aomori Apple Experimental Station, 036 Kuroishi, Japan |
| |
Abstract: | Summary DNA amplification fingerprinting using arbitrary primer(s) was applied to the identification of Malus species. Highly variable DNA fragment patterns were clearly detected by polyacrylamide gel electrophoresis of the amplified extension products, although three sports of Delicious exhibited the same fingerprint as the original cultivar. The fingerprinting of two triploid apple cultivars suggested that the parent contributing the 2n gamete was the female. We applied this fingerprinting to paternity analysis of an apple cultivar of which the pollen parent was unknown. By using 5 arbitrary primers and RFLP analysis of the amplified products, one cultivar was singled out for paternity among six putative candidates. |
| |
Keywords: | DNA fingerprint Apple Malus PCR RAPDs paternity analysis cultivar identification |
本文献已被 SpringerLink 等数据库收录! |
|