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RFLP-based mapping of the Sec-2 and Sec-5 loci encoding 75K γ-secalins of rye |
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| Authors: | S V Malyshev T O Khmyl K I Zabenkova A V Voylokov V N Korzun N A Kartel |
| Institution: | Institute of Genetics and Cytology, F. Skorina St. 27, 220074 Minsk, Belarus;St Petersburg State University, Universitetskaya nab. 7/9, 199034 St Petersburg, Russia;Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstraße 3, D-06466 Gatersleben, Germany |
| Abstract: | For mapping the Sec2 and Sec5 loci of rye which determine expression of 75K γ-secalins, a partial genetic map of chromosome 2R spanning 64 cM was constructed. The map was developed using an F2 population of 103 plants from a cross between two inbred lines. Both loci were mapped distally on the short arm of chromosome 2R and clearly tagged in relation to 12 restriction fragment length polymorphism (RFLP) markers. The Sec2 locus was localized between the Xiag57 and Xpsr109a loci in an 11 cM interval. The Sec5 locus co-segregated to Xiag57 and was tightly linked to the Sec2 locus at a map distance of 0.5cM. |
| Keywords: | Secale cereale molecular markers secalins seed storage proteins RFLP |