| 悬铃木ISSR反应体系的建立 |
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| 引用本文： | 王艳梅,景自景,刘震,田佳桂,王佳慧.悬铃木ISSR反应体系的建立[J].山东农业大学学报(自然科学版),2011,42(1). |
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| 作者姓名： | 王艳梅 景自景 刘震 田佳桂 王佳慧 |
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| 作者单位： | 1. 河南农业大学,林学院,河南,郑州,450002
2. 河南农业大学,林学院,河南,郑州,450002;北京林业大学林学院,森林培育与保护省部共建重点实验室,北京,100083 |
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| 基金项目： | 教育部留学回国人员科研启动资助项目，河南省杰出青年基金项目，教育部博士点基金项目，河南农业大学博士启动项目，河南省教育厅自然科学研究计划项目 |
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| 摘 要： | 为了探讨适合悬铃木的ISSR体系,选育无球少球悬铃木,本实验以悬铃木幼嫩叶片为实验材料,通过对CTAB方法的改良,摸索出适于悬铃木基因组DNA提取的方法,并以核酸纯度、片断分布情况等指标来评价,获得高质量基因组DNA;用2个不同样品对16对ISSR引物进行了筛选,并应用其中的4对ISSR引物对15株悬铃木进行了初步应用,获得了清晰稳定的扩增图谱.上述结果表明获得的基因组质量较高,同时也表明反映体系对悬铃木的ISSR分析是可行的;并对影响悬铃木基因组DNA的制备及扩增反应因素进行了简要的分析讨论.
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| 关 键 词： | 悬铃木 无球少球 |
ESTABLISHMENT OF ISSR REACTION SYSTEM OF PLATANUS ACERIFOLIA |
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| WANG Yan-mei,JING Zi-jing,LIU Zhen,TIAN Jia-gui,WANG Jia-hui.ESTABLISHMENT OF ISSR REACTION SYSTEM OF PLATANUS ACERIFOLIA[J].Journal of Shandong Agricultural University,2011,42(1). |
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| Authors: | WANG Yan-mei JING Zi-jing LIU Zhen TIAN Jia-gui WANG Jia-hui |
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| Institution: | WANG Yan-mei1,JING Zi-jing1,LIU Zhen1,2,TIAN Jia-gui1,WANG Jia-hui1 (1.Forestry College,HenanAgricultural University,Zhengzhou 450002,Key Laboratory for Silviculture and Conservation,Ministry of Education,Forestry College,2 Beijing Forestry University,Beijing 100083,China) |
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| Abstract: | ToprobetheeffectiveISSR system and to breed none-or-little conesPlatanus acerifolia,genomic DNA was extracted with modified CTAB methodfromtender leaves of Platanus acerifolia.DNA quality was evaluated by indexes like purityand distribution of nucleic acid.High-quality genomic DNA was obtained.Sixteen ISSR primers were screenedwith 2 different samples.4 ISSR primers were adoptedto evaluate preliminaryinitially among 15 plane treesand clear and stable amplification patterns was obtained,which indicated the D... |
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| Keywords: | ISSR |
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