| 黄连水提物抗甲型流感病毒活性初步研究 |
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| 引用本文： | 马卫列,;罗芬,;李锦云,;陈潇楷,;林东子,;丁航,;张志珍.黄连水提物抗甲型流感病毒活性初步研究[J].湛江医学院学报,2014(6):759-762. |
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| 作者姓名： | 马卫列 ;罗芬 ;李锦云 ;陈潇楷 ;林东子 ;丁航 ;张志珍 |
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| 作者单位： | [1]广东医学院生物化学与分子生物学教研室,广东东莞523808; [2]海南通用三洋药业有限公司,海南海口570312; [3]广东省东莞市慢性病防治院检验科,广东东莞523008 |
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| 基金项目： | 广东省自然科学基金项目（No.S2011010002984）;广东省高等学校人才引进专项资金项目（粤财教[2013]246号）;东莞市高等院校科研机构科技计划项目经费资助（No.2011108101007） |
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| 摘 要： | 目的观察黄连水提物对甲型流感病毒（IAV）活性的影响。方法 CCK-8法检测黄连水提物对狗肾传代（MDCK）细胞的毒性作用。采用反向遗传8质粒病毒拯救系统制备IAV后,CCK-8和CPE法检测黄连水提物抗IAV活性。利用荧光素酶编码基因报告系统研究黄连水提物对IAV感染性和RNA聚合酶活性的影响。结果黄连水提物在0.25~2.0g/L质量浓度内,对MDCK细胞有一定安全性。黄连水提物有较好的抗IAV作用。与对照组比较,黄连水提物处理组细胞的相对荧光强度明显降低（P〈0.01）。结论黄连水提物对IAV活性及感染性有较强抑制作用,可能与抑制病毒RNA聚合酶活性有关。
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| 关 键 词： | 黄连 甲型流感病毒 抗病毒活性 |
Effect of Coptis chinensis aqueous extract on activity of influenza A virus |
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| Institution: | MA Wei-lie,LUO Fen,LI Jin-yun,CHEN Xiao-kai,LIN Dong-zi,DING Hang,ZHANG Zhi-zhen(1. Department of Biochemistry and Molecular Biology, Guangdong Medical College, Dongguan 523808, China) |
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| Abstract: | Objective To study the effect of Coptis chinensis aqueous extract（CCAE） on the activity of influenza A virus（IAV）. Methods The cytotoxicity of CCAE on Madin darby canine kidney（MDCK） cells was detected by CCK-8 assay. Antiviral activity of CCAE was determined by CCK-8 and cytopathic effect methods after recombinant IAV plasmid was constructed using reverse genetic 8-plasmid system. Effects of CCAE on viral infectivity and RNA polymerase were analyzed by luciferase reporter system. Results CCAE was safe for MDCK cells at concentrations of 0.25~2.0 g/L. CCAE showed obvious anti- IAV activity. Relative fluorescence intensity of CCAE-treated cells was lower than that of controls（P〈0.01）. ConclusionCCAE can suppress the IAV activity and infectivity, which might be related to RNA polymerase inhibition. |
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| Keywords: | Coptis chinensis influenza A virus antiviral activity |
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