| 饲料糖脂比对吉富罗非鱼生长、血液指标和肝脏糖代谢关键酶活性及基因表达的影响 |
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| 引用本文： | 杨丽萍,郑文佳,秦超彬,高风英,谢帝芝,闫潇,朱华平,卢迈新,聂国兴. 饲料糖脂比对吉富罗非鱼生长、血液指标和肝脏糖代谢关键酶活性及基因表达的影响[J]. 水产学报, 2016, 40(9): 1376-1386. DOI: 10.11964/jfc.20160410370 |
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| 作者姓名： | 杨丽萍 郑文佳 秦超彬 高风英 谢帝芝 闫潇 朱华平 卢迈新 聂国兴 |
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| 作者单位： | 1. 农业部热带亚热带水产资源利用与养殖重点实验室,广东广州 510380;河南师范大学水产学院,河南新乡453007;河南省水产动物养殖工程技术研究中心,河南新乡453007;2. 河南师范大学水产学院,河南新乡453007;河南省水产动物养殖工程技术研究中心,河南新乡453007;3. 农业部热带亚热带水产资源利用与养殖重点实验室,广东广州,510380 |
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| 基金项目： | 农业部热带亚热带水产资源利用与养殖重点实验室开放基金；河南省高校科技创新团队支持计划（14IRTSTHN013）；现代农业产业技术体系专项（CARS-49） |
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| 摘 要： | 为探讨糖脂比对吉富罗非鱼幼鱼生长性能、血液生化指标和肝脏糖代谢关键酶的影响,本实验设计了5种不同糖脂比(1.5、2.3、3.9、7.0、16.5)的等氮等能饲料,以吉富罗非鱼幼鱼为实验对象,进行了为期8周的饲养实验。结果显示,随饲料中糖脂比升高,吉富罗非鱼特定生长率(SGR)、增重率(WGR)和蛋白质效率(PER)先升高后降低,糖脂比为3.9和7.0时,WGR、SGR和PER最高。饵料系数(FCR)的变化趋势与此相反,在糖脂比为3.9和7.0组显著低于其他组。随着饲料糖脂比的增加,吉富罗非鱼全鱼粗脂肪含量逐渐下降,在糖脂比为16.5时达到最低水平,但仅与1.5组存在显著差异。血浆甘油三酯在糖脂比最高组也出现显著降低,血浆胆固醇同样在糖脂比最高组显著低于3.9组。然而血糖的变化趋势相反,当糖脂比为16.5时显著高于其他组。随着糖脂比的升高,吉富罗非鱼幼鱼肝脏中丙酮酸激酶(PK)的活性及mRNA表达量逐渐升高,而磷酸烯醇式丙酮酸羧激酶(PEPCK)的活性及mRNA表达水平则逐渐下降。根据二次回归模型得出,在等氮等能的饲料条件下,吉富罗非鱼幼鱼最适宜的糖水平和脂肪水平分别为49.69%和9.53%,其对应的糖脂比为8.4。
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| 关 键 词： | 吉富罗非鱼 糖脂比 生长 血清学指标 PK PEPCK |
| 收稿时间： | 2016-04-21 |
| 修稿时间： | 2016-06-06 |
Effects of dietary carbohydrate-to-lipid ratios on growth, blood biochemical indices, key enzymes activity and gene expression of hepatic glucose metabolism of juvenile GIFT Oreochromis niloticus |
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| YANG Liping,ZHENG Wenji,QIN Chaobin,GAO Fengying,XIE Dizhi,YAN Xiao,ZHU Huaping,LU Maixin and NIE Guoxing. Effects of dietary carbohydrate-to-lipid ratios on growth, blood biochemical indices, key enzymes activity and gene expression of hepatic glucose metabolism of juvenile GIFT Oreochromis niloticus[J]. Journal of Fisheries of China, 2016, 40(9): 1376-1386. DOI: 10.11964/jfc.20160410370 |
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| Authors: | YANG Liping ZHENG Wenji QIN Chaobin GAO Fengying XIE Dizhi YAN Xiao ZHU Huaping LU Maixin NIE Guoxing |
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| Affiliation: | Key Laboratory of Tropical & Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Guangzhou 510380, China;College of Fisheries, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Guangzhou 510380, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, China,College of Fisheries, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Guangzhou 510380, China,Key Laboratory of Tropical & Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Guangzhou 510380, China and College of Fisheries, Henan Normal University, Xinxiang 453007, China;Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, China |
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| Abstract: | The purpose of this study was to evaluate the effects of dietary carbohydrate-to-lipid (CHO:LIP) ratios on growth performance, plasma biochemical indexes, pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK) activity and mRNA expression in liver of juvenile GIFT Oreochromis niloticus. Five isonitrogenous and isoenergetic diets were formulated to contain various CHO:LIP ratios ranging from 1.5 to 16.5. Each diet was tested in three replicates of fish[initial weight:(6.77±0.14) g] for 8 weeks in the recirculating aquacultural system. The results indicated that specific growth rate (SGR), weight gain rate (WGR), protein efficiency ratio (PER) increased significantly as dietary CHO:LIP varied from 3.9 to 7.0, while feed conversion ratio (FCR) showed the opposite variation trend. Crude lipid content of whole body decreaced as dietary CHO:LIP ratios increased, and was at the lowest level in 16.5 group, which was significantly decreased only compared with 1.5 group. Plasma concentrations of triglycerides and total cholesterol also significantly decreased in 16.5 group. However, serum glucose increased significantly as CHO:LIP ratios increased. High dietary carbohydrate significantly enhanced the activities and mRNA expression of liver PK, while the effects of high carbohydrate on the activity and mRNA level of liver PEPCK were in a contrary tendency. Based on the second-order polynomial regression analysis of SGR, the optimal dietary carbohydrate and lipid contents for fingerling GIFT O. niloticus were 49.69% and 9.53%, respectively, with a corresponding dietary CHO:LIP ratio of 8.4. |
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| Keywords: | GIFT Oreochromis niloticus Carbohydrate-to-lipid ratio growth serum indices PK PEPCK |
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