向日葵抗锈病基因同源序列的克隆与分析

向日葵锈病严重影响向日葵的产量。为了找到抗锈病相关基因，根据已知NBS-LRR型抗病基因保守结构域P-loop和GLPL设计简并引物，以接菌的抗病向日葵品种CM29叶片的cDNA为模板进行扩增。通过克隆转化得到10个具有连续开放阅读框的RGA，经系统进化分析将其分为TIR-NBS-LRR和non-TIR-NBS-LRR两种类型。对其氨基酸序列进行多重序列比对结果显示所获得的RGA具有典型的NBS-LRR型抗病基因保守结构域即P-loop、kinase-2a、kinase-3a和GLPL结构。运用BLASTX分析这些结果，表明其RGA与已知的抗病基因相应保守区域的同源性为18.1%～51.1%，说明它们可能与抗病功能基因具有密切联系。

Cloning and analysis of rust resistance gene analogs of sunflower

Sunflower (Helianthus annuus) rust restricts growth and production severely. In order to find resistance genes of sunflower to rust disease, degenerated primers were designed based on conserved regions of NBS-LRR genes, P-loop and GLPL, to clone the analogs from infected resistant cultivar CM29. By phylogenetic analysis, 10 cloned resistance gene analogs with continuous open reading frame belonged to TIR-NBS-LRR type and non-TIR-NBS-LRR. Multiple sequence alignment of amino acid showed these RGAs had typical domains of NBS-LRR resistance genes, including P-loop, kinase-2a, kinase-3a and GPLP. These RGAs showed great homology with the known resistance genes by BLASTX analysis, indicating the RGCs were highly related to resistance genes in sunflower.