| 水稻蛋白OsOlel在大肠杆菌中的诱导表达 |
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| 引用本文： | 姚清国,李小兵,韩爱云. 水稻蛋白OsOlel在大肠杆菌中的诱导表达[J]. 安徽农业科学, 2011, 0(19): 11399-11400 |
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| 作者姓名： | 姚清国 李小兵 韩爱云 |
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| 作者单位： | 石家庄学院化工学院; |
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| 基金项目： | 石家庄市科学技术研究与发展课题(10120803); 石家庄学院科研启动基金(2007012) |
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| 摘 要： | [目的]在大肠杆菌中诱导表达水稻蛋白OsOle1。[方法]RT-PCR克隆水稻基因OsOle1,构建原核表达载体pGEX-2T-OsOle1-GST,在大肠杆菌BL21中诱导表达该蛋白,并采用GST凝胶亲和层析进行纯化。[结果]水稻蛋白OsOle1具有典型的Olee1蛋白家族的保守域,N端1～24位氨基酸为预测的信号肽序列。成功地将水稻蛋白OsOle1在大肠杆菌中进行了诱导表达,并用GST亲和层析进行了纯化。[结论]该研究为以后进一步研究水稻蛋白OsOle1的生理功能奠定了基础。
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| 关 键 词： | 水稻 OsOlel 诱导表达 亲和层析 |
Induced Expression of Rice Protein OsOle1 in Escherichia coli |
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| YAO Qing-guo et al. Induced Expression of Rice Protein OsOle1 in Escherichia coli[J]. Journal of Anhui Agricultural Sciences, 2011, 0(19): 11399-11400 |
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| Authors: | YAO Qing-guo et al |
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| Affiliation: | YAO Qing-guo et al(College of Chemical Engineering,Shijiazhuang University,Shijiazhuang,Hebei 050035) |
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| Abstract: | [Objective]The research aimed to induce the expression of rice protein OsOle1 in Escherichia coli.[Method]RT-PCR was used to clone OsOle1 in the rice,and the prokaryotic expression vector pGEX-2T-OsOle1-GST was constructed.The protein was induced expressing in Escherichia coli BL21,and GST gel affinity chromatography was used to purify.[Result]The rice protein OsOle1 had the typical conserved domain of Olee1 protein family.The amino acids at 1-24 sites in the N terminal were the predicted signal peptide seq... |
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| Keywords: | Rice OsOle1 Induced expression Affinity chromatography |
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