| 紫花月季组织培养与快速繁殖研究 |
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| 引用本文： | 林小洁,李洪清. 紫花月季组织培养与快速繁殖研究[J]. 安徽农业科学, 2011, 0(19): 11380-11381,11385 |
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| 作者姓名： | 林小洁 李洪清 |
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| 作者单位： | 广东省阳江职业技术学院;华南师范大学生命科学院广东省植物发育生物工程重点实验室; |
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| 基金项目： | 广东省植物发育生物工程重点实验室开放课题基金项目 |
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| 摘 要： | [目的]建立适宜的紫花月季[Rosa chinensis Jacq.var.semperflorens（Curtis）Koehne]无性繁殖体系。[方法]以紫花月季茎为外植体,对适合月季丛生芽诱导的培养基和切取外植体的方式进行优化筛选。[结果]芽诱导的最适培养基为MS＋1.0mg/L6-BA＋0.2mg/L GA3;丛生芽增殖的适宜培养基为MS＋3.0mg/L6-BA＋0.1mg/L NAA;再生芽在MS＋0.5mg/L NAA培养基中生根效果最好。1个侧芽在经历21d丛生芽诱导和28d芽增殖培养后,可获得90多个再生芽。[结论]该研究可为实现紫花月季育苗的规模化和产业化生产提供参考。
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| 关 键 词： | 紫花月季 组织培养 离体快繁 |
Study on Tissue Culture and Rapid Propagation of R.chinensis Jacq.var.semperflorens (Curtis) Koehne |
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| LIN Xiao-jie et al. Study on Tissue Culture and Rapid Propagation of R.chinensis Jacq.var.semperflorens (Curtis) Koehne[J]. Journal of Anhui Agricultural Sciences, 2011, 0(19): 11380-11381,11385 |
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| Authors: | LIN Xiao-jie et al |
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| Affiliation: | LIN Xiao-jie et al(Guangdong Provincial Yangjiang Vocational & Technical College,Yangjiang,Guangdong 529566) |
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| Abstract: | [Objective]The aim was to establish a suitable asexual propagation system of R.chinensis Jacq.var.semperflorens(Curtis) Koehne.[Method]Using stems as explants,the suitable media for inducing cluster buds of R.chinensis Jacq.var.semperflorens(Curtis) Koehne and the method of cutting explants were optimized and screened.[Result]The optimal media for induction and proliferation of multiple buds were MS + 1.0 mg/L 6-BA + 0.2 mg/L GA3 and MS + 3.0 mg/L 6-BA + 0.1 mg/L NAA,respectively.Regenerated shoots rooted e... |
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| Keywords: | R.chinensis Jacq.var.semperflorens(Curtis) Koehne Tissue culture In vitro propagation |
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