玉米花药培养影响因素研究

采用36种玉米基因型材料,研究了基因型、培养基、接种前冷藏以及田间早期对雄穗注射一定浓度的2,4-D和在接种前用一定浓度的2,4-D浸泡雄穗对出愈率的影响。结果表明:在玉米培养基 N6(微量元素) 2,4-D2mg/L KT2mg/L 水解酪蛋白(CH)500mg/L 活性炭0.2% 糖12% 琼脂6.5g的诱导培养基中,36个基因型玉米中只有7个基因型能诱导出愈伤组织。其中博丰5号在接种前用4℃冷藏7d出愈率可增加3.71%;SB304在13～14叶期用2,4-D注射雄穗能有效提高其出愈率;金象4号雄穗在接种前用2,4-D浸泡,随着2,4-D浓度的增加或浸泡时间的加长,其出愈率都在增加。

Research of the Effective Factors on Maize Anther Culture

Countering the unresolved problem of maize anther culture on the low induction frequency,36 varieties cone used as the materials,effects of factors such as plant genotypes,medium,low temperature pretreatment and injecting 2,4-D on male spikelet at field or soaking male spikelet in 2,4-D at lab were studied in the experiment.The results showed that using cone media and little chemical element of N6,2 mg/L 2,4-D,2 mg/L KT,500 mg/L CH,0.2% AC,12% sugar as initiation medium,only 7 genotypes in 36 varieties cone could get callus.In the 7 varieties cone,the callus frequency of Bo feng No.5 could be increased 3.71% after seven days in low temperature before inoculating,injecting 2,4-D on male spikelet of SB304 at 13-14 leaves could raise the rate of callus.Another,male spikelet of Jing-xiang No4 was soaked in 2,4-D for some tines,the dense been increased of 2,4-D or the soaking time been lengthened could both raise the rate of the callus.