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Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers |
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| Authors: | Wen-zheng CHAO Chuan-hong TANG Jing-song ZHANG Ling YU Honda Yoichi |
| Institution: | 1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, P.R.China;2. Key Laboratory of Edible Fungus Resources and Utilization (South), Ministry of Agriculture/National Engineering Research Center of Edible Fungi/National R&D Center for Edible Fungi Processing/Key Laboratory of Agriculture Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R.China;3. Laboratory of Forest Biochemistry, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan |
| Abstract: | The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content. This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region (SCAR) marker for rapid identification of the G. lucidum Hunong 5 cultivar. The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat (ISSR) fragment (882 bp) from G. lucidum Hunong 5 cultivar. A stable SCAR primer pair GLH5F/GLH5R were obtained to identify the cultivar and the SCAR marker is a DNA fragment of 773 bp. |
| Keywords: | DNA pooling Hunong 5 cultivar ISSR marker SCAR marker |
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