| 筛选水稻贮藏蛋白突变体的方法研究 |
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| 引用本文： | 江绍玫,朱速松,沈文飚,郑天清,王益华,江玲,王春明,徐朗莱,万建民. 筛选水稻贮藏蛋白突变体的方法研究[J]. 南京农业大学学报, 2003, 26(4): 17-20 |
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| 作者姓名： | 江绍玫 朱速松 沈文飚 郑天清 王益华 江玲 王春明 徐朗莱 万建民 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室,江苏省植物基因工程技术研究中心,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金资助项目 (3 0 170 5 70 )，中国水稻发展基金资助项目 (0 0 0 3 10 2 )，江苏省自然科学基金资助项目(BJ2 0 0 0 0 19) |
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| 摘 要： | 根据水稻种子及其贮藏蛋白特性 ,结合普通实验室条件 ,对分离水稻种子全蛋白及其各蛋白组分的方法进行改进 ,建立了筛选水稻种子贮藏蛋白突变体的新方法。讨论了抽提水稻种子全蛋白时试剂加入的最佳方式以及各蛋白组分分级分离时的最佳条件。新方法简便易行 ,快速高效 ,重复性好。将该法用于种子资源库筛选 ,得到了较满意的结果。
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| 关 键 词： | 水稻 贮藏蛋白 突变体 筛选方法 |
| 文章编号： | 1000-2030(2003)04-0017-04 |
| 修稿时间： | 2003-03-16 |
Research on methods for screening rice mutants of storage proteins |
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| JIANG Shao-mei,ZHU Su-song,SHEN Wen-biao,ZHENG Tian-qing,WANG Yi-hua,JIANG Ling,WANG Chun-ming,XU Lang-lai,WAN Jian-min. Research on methods for screening rice mutants of storage proteins[J]. Journal of Nanjing Agricultural University, 2003, 26(4): 17-20 |
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| Authors: | JIANG Shao-mei ZHU Su-song SHEN Wen-biao ZHENG Tian-qing WANG Yi-hua JIANG Ling WANG Chun-ming XU Lang-lai WAN Jian-min |
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| Affiliation: | JIANG Shao-mei,ZHU Su-song,SHEN Wen-biao,ZHENG Tian-qing,WANG Yi-hua,JIANG Ling,WANG Chun-ming,XU Lang-lai,WAN Jian-min* |
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| Abstract: | A new method was developed to screen rice mutants of storage proteins by improving the old one for isolating total proteins in seeds,based on the characteristic of seeds and storage proteins in rice and condition in general laboratories.The optimal experimental conditions for isolating storage proteins in rice were surveyed.In comparision with the old method,the new one is more simple,efficient and repeatable.Certain ideal results were obtained by analyzing the total proteins in rice resources seeds through the application of this method. |
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| Keywords: | rice storage proteins mutant screening method |
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