| 重叠区扩增法合成抗菌肽B基因 |
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| 引用本文： | 桑春果,张开春,张军科. 重叠区扩增法合成抗菌肽B基因[J]. 西北农林科技大学学报(自然科学版), 2002, 30(1): 65-67 |
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| 作者姓名： | 桑春果 张开春 张军科 |
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| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西,杨陵,712100
2. 北京市农林科学院,林果所,北京,100093 |
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| 基金项目： | 北京市科委合同项目 ( 85 5 130 40 0 ) |
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| 摘 要： | 人工设计并合成了抗菌肽 B基因的 4个寡聚核苷酸片段 ,通过重叠区扩增法 ,扩增出了相当于抗菌肽基因全长的寡聚核苷酸片段。经克隆测序 ,证明成功地实现了抗菌肽基因的人工合成、拼接和克隆。
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| 关 键 词： | 重叠区扩增法 抗菌肽 基因合成 基因克隆 |
| 文章编号： | 1000-2782(2002)01-0065-03 |
| 收稿时间： | 2001-09-17 |
| 修稿时间： | 2001-09-17 |
Synthesis and cloning of antibacterial peptide B (cecropin B) gene by overlap extension |
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| YANG Ya-ti,ZHANG Yi-ping,ZHANG Xing-fu. Synthesis and cloning of antibacterial peptide B (cecropin B) gene by overlap extension[J]. Journal of Northwest A&F University（Natural Science Edition）, 2002, 30(1): 65-67 |
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| Authors: | YANG Ya-ti ZHANG Yi-ping ZHANG Xing-fu |
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| Affiliation: | 1 (1 College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China; 2 Institute of Pomology and Forestry,Beijing Academy of Agriculture and Forestry,Beiji |
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| Abstract: | Designed and synthesised 4 fragments of antibacterial peptide B gene,then these fragments were combinated into one expected fragment by the method of gene splicing by overlap extension (gene SOEing).The interested fragment was cloned and sequenced and showed a good result as expected. |
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| Keywords: | gene splicing by overlap extension antibacterial peptide gene synthesis gene cloning |
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