| 烟草叶绿体16S启动子的克隆改造及转基因植株的获得 |
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| 引用本文： | 山松,张中林.烟草叶绿体16S启动子的克隆改造及转基因植株的获得[J].作物学报,1999,25(5):536-540. |
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| 作者姓名： | 山松 张中林 |
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| 作者单位： | 中国农业科学院生物技术研究中心，北京100081 |
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| 基金项目： | 本研究系国家科委风险基金资助. |
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| 摘 要： | 设计引物并克隆了烟草叶绿体16SrDNA基因的启动子，并在其下游加入了rbcL基因的SD序列，以使该启动子能有效翻译外源基因；进一步以aadA基因作为外源基因，以psbA基因的3’序列为终止子，构建成aadA基因表达盒；将此表达盒插入烟草叶绿体同源片段trnK-psbA-trnH中，构建成烟草叶绿体转化及表达载体p16T。用基因枪微弹轰击转化
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| 关 键 词： | 烟草 16S启动子 基因枪转化法 叶绿体表达体系 |
| 收稿时间： | 1998-12-23 |
| 修稿时间： | 1999-05-15 |
Cloning and Modification of 16S Promoter of Tobacco Chloroplast Genome and Production of Transgenic Plants |
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| Shan Song Zhang Zhonglin Chen Xi Liu Chunying Shen Guifang.Cloning and Modification of 16S Promoter of Tobacco Chloroplast Genome and Production of Transgenic Plants[J].Acta Agronomica Sinica,1999,25(5):536-540. |
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| Authors: | Shan Song Zhang Zhonglin Chen Xi Liu Chunying Shen Guifang |
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| Institution: | Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, 100081 |
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| Abstract: | A Nicotiana tabacum chloroplast transformation vector p16T has been constructed on the basis of a mutated 16S rDNA promoter. It contains not only aadA. gene regulated by 16S promoter and psbA teminator, but also two homologous fragments, trnH-psbA and trnK. Using biolistic method, the plasmid p16T has been transformed into the wild type Nicotiana tabacum leaves. Through spectinomycin selection, transformed calli and regenerated plants were obtained from tobacco seedling leaves. The result of PCR analysis showed that the foreign gene had been transformed into the chloroplast of tobacco. Hybrid seedlings of transgenic tobacco acting as the female parent showed the resistance to spectinomycin with the mode of maternal heredity. |
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| Keywords: | Nicotiana tdbacum 165 promoter Chloroplast expression system Biolistic trans-formation |
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