| 麦冬花药离体培养与再生植株的获得 |
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| 引用本文： | 王化东,吴发明.麦冬花药离体培养与再生植株的获得[J].湖北农业科学,2012,51(12):2602-2603. |
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| 作者姓名： | 王化东 吴发明 |
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| 作者单位： | 1. 四川中医药高等专科学校,四川绵阳,621000
2. 成都中医药大学药学院/中药材标准化教育部重点实验室,成都,611137 |
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| 摘 要： | 以麦冬Ophiopogon japonicus (Thunb.)Ker-Gawl.]花药为材料,将其接种在培养基上诱导愈伤组织,再将愈伤组织在分化培养基上培养,将分化所得的再生植株炼苗后移栽大田.结果表明,各个培养基配方及试验条件适宜,麦冬愈伤组织诱导较为成功,愈伤组织的分化效果也较好,可达41.5％,组培苗移栽后生长良好,成活率达80％以上.
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| 关 键 词： | 麦冬[Ophiopogon japonicus (Thunb.)Ker-Gawl.] 花药 组织培养 |
Research on in vitro Culture of Ophiopogon japonicus Anther and Plant Regeneration |
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| WANG Hua-dong
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WU Fa-ming.Research on in vitro Culture of Ophiopogon japonicus Anther and Plant Regeneration[J].Hubei Agricultural Sciences,2012,51(12):2602-2603. |
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| Authors: | WANG Hua-dong WU Fa-ming |
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| Institution: | 1.Sichuan College of Traditional Chinese Medicine,Mianyang 621000,Sichuan,China;2.Chengdu University of Traditional Chinese Medicine/The Ministry of Education Key Laboratory of Standardization of Chinese Crude Drug,Chengdu 611137,China) |
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| Abstract: | Anther of Ophiopogon japonicus was used as materials to induce callus.The callus was then cultured in differentiation medium to obtain regeneration plant of O.japonicus.After plantlet training,the seedlings were then transplanted in field.The results showed that formula of medium and experimental conditions were appropriated;callus induction of somatic embryos and differentiation was very successful with a differentiation rate of 41.5%.Transplanted seedlings grew well as the survival rate was above 80%. |
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| Keywords: | Ophiopogon japonicus(Thunb )Ker-Gawl anther tissue culture |
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