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猪苓菌核与猪苓菌丝体中提取多糖对小鼠免疫器官重量影响的比较研究
引用本文:田广燕,李太元,许广波,金庆日,韩贞珍.猪苓菌核与猪苓菌丝体中提取多糖对小鼠免疫器官重量影响的比较研究[J].延边大学农学学报,2005,27(2):83-86.
作者姓名:田广燕  李太元  许广波  金庆日  韩贞珍
作者单位:延边大学农学院,动物医学系,吉林,龙井,133400
基金项目:国家自然科学基金项目(30460084),吉林省科技厅项目(20000203-3-2)
摘    要:通过人工液体浅层培养的方法成功培养出猪苓菌丝体.用热水浸提法分别提取猪苓菌核和猪苓菌丝体中的多糖,并用3,5一二硝基水杨酸法测定其糖含量,进一步纯化处理后,用傅里叶红外吸收光谱仪进行比较分析表明,两者红外光谱图相似.通过对小鼠免疫器官重量的免疫学检测,表明从猪苓菌丝体中提取的多糖和从猪苓菌核中提取的多糖均有提高机体免疫器官重量的作用,统计学分析表明差异不显著。

关 键 词:猪苓菌丝体  猪苓菌核  猪苓多糖  免疫
文章编号:1004-7999(2005)02-0083-04
修稿时间:2005年3月15日

Effects of polysaccharose extracted from sclerotium and mycelium of Polyporus umbellatus (Pers.)Fr. on the weight of immunological organs of mice
TIAN Guang-yan,LI Tai-yuan,XU Guang-bo,JIN Qing-ri,HAN Zhen-zhen.Effects of polysaccharose extracted from sclerotium and mycelium of Polyporus umbellatus (Pers.)Fr. on the weight of immunological organs of mice[J].Journal of Agricultural Science Yanbian University,2005,27(2):83-86.
Authors:TIAN Guang-yan  LI Tai-yuan  XU Guang-bo  JIN Qing-ri  HAN Zhen-zhen
Institution:TIAN Guang-yan,LI Tai-yuan~*,XU Guang-bo,JIN Qing-ri,HAN Zhen-zhen
Abstract:The mycelia of Polyporus umbellatus (Pers.) Fr. were cultured by superficial solution culture. The polysaccharose of sclerotium and mycelia of Polyporus umbellatus (Pers.) Fr. were extracted by hot water and their contents were measured by 3,5-dinitro-salicylate method. The infrared spectrograms of the two kinds of polysaccharose had similar infrared spectrograms analyzed by infrared absorption spectrograph after further purification. Both of them could advance the weight of immunological organs in mice and had no significant difference (measured) by the immunological measure.
Keywords:mycelium of Polyporus umbellatus (Pers  ) Fr    sclerotium of Polyporus umbellatus (Pers  ) Fr    polysaccharose of Polyporus umbellatus (Pers  ) Fr    immunity
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