| 鸡马立克氏病双价疫苗的研究 Ⅰ.马立克氏病SB-1病毒株在鸡胚皮肤细胞中的培养试验 |
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| 引用本文： | 张鹏程,毕秀纯,高宏伟,许星华,史长林,陈美云,刘筱华,孙桂欣.鸡马立克氏病双价疫苗的研究 Ⅰ.马立克氏病SB-1病毒株在鸡胚皮肤细胞中的培养试验[J].中国兽医学报,1990(2). |
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| 作者姓名： | 张鹏程 毕秀纯 高宏伟 许星华 史长林 陈美云 刘筱华 孙桂欣 |
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| 作者单位： | 吉林省兽医科学研究所
(张鹏程，毕秀纯，高宏伟，许星华)，吉林省生物制品厂
(史长林，陈美云，刘筱华)，吉林省生物制品厂(孙桂欣) |
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| 摘 要： | 以简便技术制备鸡胚皮肤(CES)细胞,进而进行了鸡马立克氏病(MD)SB-1株病毒适应于CES细胞和致CES细胞病变效应(CPE)的研究.试验表明,在形成CPE的细胞中可检出核内包涵体,MD的SB-1株病毒的CES细胞毒的蚀斑形成单位(pfu)为105.7201-7.2122/m L.本研究为以后用CES细胞增殖SB-1株病毒以代替鸡胚成纤维细胞(CEF)制造疫苗提供了依据.
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| 关 键 词： | 鸡马立克氏病 疫苗 鸡马立克氏病病毒 鸡胚皮肤细胞 培养 |
Studies of a Bivalent Vaccine against Marek's Disease I. Cultivation of SB-1 Strain of Marek's Disease Virus in Chicken Embryo Skin Cell Culture |
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| Zhang Pengcheng et al.Studies of a Bivalent Vaccine against Marek''''s Disease I. Cultivation of SB-1 Strain of Marek''''s Disease Virus in Chicken Embryo Skin Cell Culture[J].Chinese Journal of Veterinary Science,1990(2). |
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| Authors: | Zhang Pengcheng |
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| Institution: | The Veterinary Scientific Institute of Jilin |
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| Abstract: | A simple technique for preparing chicken embryo skin cell culture was developed and studies on adaptation of SB- 1 strain of Marek's disease virus to this cell system and cytopathic effect induced were further' undertaken. The experiment showed that the inclusion bodies were seen in infected cells and the plaque-forming unit was 106.7201-7.2122/mL.It provided some evidence that the cell culture reported maybe used in lieu of chicken embryo fibroblasts for preparation of Marek's disease vaccine. |
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| Keywords: | Marek's clisease Vaccine Marek's disease virus chicken embryo skin cell culture |
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