| 改良壳聚糖固定化真菌漆酶的研究 |
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| 引用本文： | 高千千,朱启忠,杨志国,杨列健,白朝阳,邓丽莉,张乐橦.改良壳聚糖固定化真菌漆酶的研究[J].北方园艺,2009(5). |
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| 作者姓名： | 高千千 朱启忠 杨志国 杨列健 白朝阳 邓丽莉 张乐橦 |
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| 作者单位： | 山东大学,威海分校,山东,威海,264209;山东大学,威海分校,山东,威海,264209;山东大学,威海分校,山东,威海,264209;山东大学,威海分校,山东,威海,264209;山东大学,威海分校,山东,威海,264209;山东大学,威海分校,山东,威海,264209;山东大学,威海分校,山东,威海,264209 |
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| 基金项目： | 国家青少年创新性资助项目，山东大学威海分校科技立项资助项目 |
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| 摘 要： | 以改良壳聚糖-壳聚糖铜为载体,戊二醛为交联剂对真菌漆酶进行了固定化研究,探讨了其固定化条件及固定化酶的部分性质.结果表明:戊二醛最适浓度为0.5%,交联最佳时间14 h,最佳给酶量约1.3 mg,固定时间12 h或0.5 h.在此条件下获得的固定化漆酶酶活回收率分别为56.4%与51.2%.与游离酶相比,固定化漆酶与作用底物邻联甲苯胺的亲和力降低,但固定化漆酶的稳定性有明显改善.最适温度分别为40℃和80℃,分别比游离酶提高10℃和50℃;90℃条件下保温5 h后固定化酶活保留率分别为57.4%和40%,而相同条件下游离酶酶活明显下降.
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| 关 键 词： | 改良壳聚糖 固定化 真菌漆酶 |
Studies on Fungal Laccase Immobilized by Improved Chitosan |
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| GAO Qian-qian,ZHU Qi-zhong,YANG Zhi-guo,YANG Lie-jian,BAI Zhao-yang,DENG Li-li,ZHANG Le-tong.Studies on Fungal Laccase Immobilized by Improved Chitosan[J].Northern Horticulture,2009(5). |
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| Authors: | GAO Qian-qian ZHU Qi-zhong YANG Zhi-guo YANG Lie-jian BAI Zhao-yang DENG Li-li ZHANG Le-tong |
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| Institution: | Shandong University at Weihai;Weihai;Shandong 264209;China |
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| Abstract: | Laccase from Amanita verng was immobilized on chitosan complexed by Cu+ by means of covalent coupling to a glutaradehyde-pretreated support.The condition for immobilizing and part of the qualities of the immobilized enzyme was studied.The optital conditions of immobilization could be specified as: 0.5% glutaradehyde crosslinked for 14 h,added about 1.3 mg free enzyme to immobilized for 12 h or 0.5 h,the recovery of which were 56.4% and 51.2%.Compared with the free enzyme,the affinity of immobilized enzymes ... |
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| Keywords: | Improved chitosan Immobilization Fungal laccase |
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