| 某规模化猪场大肠埃希菌O141的分离鉴定及药敏试验 |
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| 引用本文： | 宋勤叶,宣春龄,左玉柱,付春青.某规模化猪场大肠埃希菌O141的分离鉴定及药敏试验[J].动物医学进展,2006,27(10):88-91. |
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| 作者姓名： | 宋勤叶 宣春龄 左玉柱 付春青 |
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| 作者单位： | 河北农业大学动物科技学院,河北保定,071001;河北保定易县职教中心,河北易县,074200 |
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| 摘 要： | 从河北衡水某规模化猪场21日龄发病仔猪脾脏中分离到1株革兰氏阴性杆菌,通过菌落形态、培养特性和微量生化试验,鉴定为大肠埃希菌,应用大肠埃希菌O抗原定型血清鉴定为O141。该菌株对供试的14种抗菌药物均产生耐药性,其中多种常用抗菌药物对其完全没有抑制作用。动物致病性和毒素试验表明,该菌株在20 h内能够导致70%小鼠死亡,并产生热稳定肠毒素,为1株产肠毒素性大肠埃希菌。
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| 关 键 词： | 大肠埃希菌 O141 致病性 热稳定肠毒素 仔猪 |
| 文章编号： | 1007-5038（2006）10-0088-04 |
| 收稿时间： | 2006-07-26 |
| 修稿时间： | 2006年7月26日 |
Isolation Identification and Drug Sensitivity Test of Escherichia coli Strain O141 from Swine |
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| SONG Qin-ye,XUAN Chun-ling,ZUO Yu-zhu,FU Chun-qing.Isolation Identification and Drug Sensitivity Test of Escherichia coli Strain O141 from Swine[J].Progress In Veterinary Medicine,2006,27(10):88-91. |
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| Authors: | SONG Qin-ye XUAN Chun-ling ZUO Yu-zhu FU Chun-qing |
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| Institution: | 1. College of Animal Science and Techonology , Hebei Agricultural University, Baoding , Heibei, 071001, China ; 2. Vocational School of Yixian County, Yixian , Hebei , 074200, China |
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| Abstract: | A gram-negative bacterium was isolated from the spleen of a sick piglet at the age of 21 days in a farm in Hengshui,Hebei Province.This bacterium was identified as Escherichia coli by colony form,culture characteristic and minimal biochemical test,and its serotype as O141 by slide agglutination test.The E.coli strain appeared resistant to 14 kinds of antimicrobical drugs used in the experiment,most of which had no any inhibitive effect on this strain.Furthermore,the strain caused 70% of mice to die within 20 h and produced heat-stable enterotoxin(ST).The results indicate it is enterotoxigenic E.coli. |
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| Keywords: | O141 |
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