壳聚糖-胶体金修饰丝网印刷电极测定过氧化氢

实验制作了丝网印刷酶电极，采用不同的方法对电极进行修饰，比较了壳聚糖酶电板和壳聚糖胶体金修饰酶电极对过氧化氢的响应。结果表明，在磷酸盐缓冲液（0．1mol·L^-1，pH7．2，PB）中，酶电板的二茂铁氧化峰消失，而还原峰电流高，峰形较好，在检测过程中，电极性能稳定，重复性好。当过氧化氢浓度范围在0.08-40mg·L^-1时，壳黎糖胶体金修饰酶电板对过氧化氢响应电流具有较好的线性关系，面归方程为Y=0．94066logC-1．48549（C为过氧化氢浓度），检测下限为80μg·L^-1，检测精度高，反应灵敏，而且抗干扰能力较强，适合用于食品中过氧化氢的快速检测。

Chitosan and Gold Colloid Modified Screen-Printed Electrodes for Measurement of Hydrogen Peroxide

A sensitive amperometrie screen-printed electrode modified with two different methods was developed for measurement of hydrogen peroxide. The results showed that the oxidation peak of ferrocene in enzyme electrode was disappeared, however, the reduction peak had a good shape and strong current in phosphate buffer （PB 0.1 mol·L^-1, pH7.2）. The enzyme electrode with stable performance excelled at the measurement of hydrogen peroxide. A good linear correlation between reduction current of electrode and concentration of H2O2 was manifested with the range of H2O2 0.08-40 mg·L^-1. The low limit was to 0.08 mg·mL^-1 The novel and sensitive method with high precision has a distinguished antiturbulance ability which would be suitable for the spot detection of H2O2 in food.