| 分子标记技术在寄生虫分类鉴定上的应用 |
| |
| 引用本文： | 陈俊锋,俞纯方,黄兵,韩静芳. 分子标记技术在寄生虫分类鉴定上的应用[J]. 动物医学进展, 2006, 27(5): 23-27,50 |
| |
| 作者姓名： | 陈俊锋 俞纯方 黄兵 韩静芳 |
| |
| 作者单位： | 西南大学动物科技学院,重庆,400715;中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室,上海,200232;西南大学动物科技学院,重庆,400715;中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室,上海,200232 |
| |
| 基金项目： | 科技部科技基础条件平台建设计划 |
| |
| 摘 要： | 分子标记与形态标记、细胞学标记和生化标记3类遗传标记技术相比,具有直接以DNA的形式表现,在动物的任何生长阶段都可检测,遍布整个基因组,多态性高,检测手段简单迅速,无基因多效性,能够明确辨别等位基因,试验重复性好等优点.目前,在寄生虫分类鉴定上常用的分子标记技术有RFLP,PCR-RFLP,RAPD和AFLP.文章综述了4种分子标记技术的基本原理、主要优缺点以及在寄生虫分类鉴定中的应用情况.
|
| 关 键 词： | 分子标记 RFLP PCR-RFLP RAPD AFLP 寄生虫 分类鉴定 |
| 文章编号： | 1007-5038（2006）05-0023-06 |
| 收稿时间： | 2006-03-24 |
| 修稿时间： | 2006-03-24 |
Application of Molecular Marker Technology in Classification and Identification of Parasites |
| |
| CHEN Jun-feng,YU Chun-fang,HUANG Bing,HAN Jing-fang. Application of Molecular Marker Technology in Classification and Identification of Parasites[J]. Progress In Veterinary Medicine, 2006, 27(5): 23-27,50 |
| |
| Authors: | CHEN Jun-feng YU Chun-fang HUANG Bing HAN Jing-fang |
| |
| Affiliation: | 1. College of Animal Science and Technology, Southwest University, Chongqing , 400715, China; 2. Shanghai Institute of Domestic Animal Parasitology, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture ,Shanghai, 200232, China |
| |
| Abstract: | Compared with the marker technologies in morphology, cytology and biochemistry, DNA molecular marker technology directly based on DNA with the advantages of no restriction on animal development stages, residing in the whole genomes, allelic identification with numerous polymorphisms, simple and quick detection, and well experimental repeatability. At present, some DNA-based molecular methods were used to identify the parasites, such as RFLP, PCR-RFLP, RAPD and AFLP. In this paper we reviewed the rudiment, advantages, disadvantages and its application of these methods in the identification of parasites. |
| |
| Keywords: | RFLP PCR-RFLP RAPD AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
