| 青霉植酸酶生产条件及纯化的研究 |
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| 引用本文： | 何平,傅雪琳,詹福建,赵亚华,高向阳,许少杰. 青霉植酸酶生产条件及纯化的研究[J]. 华南农业大学学报, 2002, 23(2): 44-46 |
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| 作者姓名： | 何平 傅雪琳 詹福建 赵亚华 高向阳 许少杰 |
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| 作者单位： | 华南农业大学生命科学学院,广东,广州,510642;华南农业大学农学院,广东,广州,510642 |
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| 基金项目： | 华南农业大学校长基金资助项目 (990 0 3) |
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| 摘 要： | 青霉培养液的原始pH值为5.5时,植酸酶大量地产生,酶活性高,在青霉的生长过程中,维持培养液pH的不降低会抑制青霉的生长,植酸钙可促进植酸酶活性的提高,粗酶液经硫酸铵分级沉淀,再经SephadexG-100凝胶过滤,洗脱液有2个具有酶活力的蛋白峰,植酸酶被纯化了3.3倍,比活力达71.3U/mg。
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| 关 键 词： | 青霉 植酸 植酸酶 |
| 文章编号： | 1001-411X(2002)02-0044-03 |
| 修稿时间： | 2001-09-18 |
Studies on the Condition of Phytase Produced by Penicillium and Its Purification |
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| HE Ping,FU Xue lin,ZHAN Fu jian,ZHAO Ya hu,GAO Xiang yang,XU Shao jie. Studies on the Condition of Phytase Produced by Penicillium and Its Purification[J]. JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY, 2002, 23(2): 44-46 |
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| Authors: | HE Ping FU Xue lin ZHAN Fu jian ZHAO Ya hu GAO Xiang yang XU Shao jie |
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| Affiliation: | HE Ping 1,FU Xue lin 2,ZHAN Fu jian 1,ZHAO Ya hua 1,GAO Xiang yang 1,XU Shao jie 1 |
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| Abstract: | When the original pH value of the penicillium's culture fluid was 5.5,the phytase was produced much .While the pH of culture fluid didn't decrease, the growth of penicillium could be inhibited. Calcium phytate could promote the penicillium to increase phytase activities.Two isoenzymes of phytase from penicillium were accepted by ammonium sulfate precipitation,sephadex G-100 gel filtration chromatography. The purification factors of phytase reached 3.3 folds with a specific activity of 71 3 U/mg. |
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| Keywords: | penicillium phytate phytase |
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