| 甘蔗茎尖胚状体脱毒苗快繁技术研究 |
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| 引用本文： | 李松,余坤兴,刘丽敏,淡明,刘红坚,杨柳,谭芳,游建华,戴友铭.甘蔗茎尖胚状体脱毒苗快繁技术研究[J].中国糖料,2010(4). |
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| 作者姓名： | 李松 余坤兴 刘丽敏 淡明 刘红坚 杨柳 谭芳 游建华 戴友铭 |
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| 作者单位： | 中国农业科学院甘蔗研究中心;广西壮族自治区甘蔗研究所;广西大学; |
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| 基金项目： | 国家科技支撑计划项目，广西区科技计划项目，广西甘蔗研究所基本科研业务专项课题 |
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| 摘 要： | 以甘蔗新台糖22号为材料,比较茎尖胚状体、茎尖与腋芽3种分化成苗繁育方法在不同时期接种、不同激素水平下的组培苗增殖速度、苗素质及脱毒效果。试验结果表明:组培苗繁殖速度以茎尖胚状体分化苗最快,增殖5代后扩繁2589倍,茎尖297倍,腋芽104倍,培养基以6-BA 1.5mg/L+NAA 0.01~0.1mg/L增殖效果最好;组培苗质量与繁殖速度相反;出现不正常生长的苗类型有白化苗、细弱小苗、玻璃化苗、疯长苗4种,茎尖胚状体苗发生率1.77%,茎尖苗1.56%,腋芽苗0.31%;不同处理间组培苗生根及移栽成活率差异不显著,生根率茎尖胚状体苗75.3%、茎尖苗76.9%、腋芽苗76.6%,移栽成活率茎尖胚状体苗94.8%、茎尖苗95.4%、腋芽苗95.1%,生根培养基以NAA7.5mg/L+ABA 2.5mg/L最好;去除RSD、花叶病方面,以茎尖胚状体苗最好,RSD去除率95%、花叶病去除率100%,茎尖苗RSD去除率70%、花叶病75%,腋芽苗未能去除RSD、花叶病。应用茎尖胚性细胞再生植株,脱毒效果好,繁殖速度快,可克服目前脱毒苗生产中试管苗扩繁量小、成本高的难题。
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| 关 键 词： | 甘蔗 茎尖 胚状体 脱毒 |
Sugarcane Micropropagation through Shoot-tip Embryogenesis |
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| LI Song,YU Kun-xing,LIU LI-min,DAN Ming,LIU Hong-jian,YANG Liu,et al.Sugarcane Micropropagation through Shoot-tip Embryogenesis[J].Sugar Crops of China,2010(4). |
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| Authors: | LI Song YU Kun-xing LIU LI-min DAN Ming LIU Hong-jian YANG Liu |
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| Institution: | LI Song1,2,YU Kun-xing1,LIU LI-min1,DAN Ming1,LIU Hong-jian1,YANG Liu3,et al(1.Sugarcane Research Center,Chinese Academy of Agricultural Sciences,Nanning 53007,China,2.Guangxi Sugarcane Research Institute,3.Guangxi University,Nanning 530005,China) |
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| Abstract: | Sugarcane ROC22 was selected as the experimental material;multiplication rate,plantlet's quality and effect of virus-free were compared between different time for inoculating and different concentration of hormones of sugarcane plantlets from embryogenesis,shoot tips and apical buds.After 5 subcultures on MS + 6-BA 1.5mg/L + NAA 0.01-0.1 mg/L medium generated 2589 plantlets from embryogenesis,297 plantlets from shoot tips and 104 plantlets from apical bud.Quality of plantlets opposite to multiplication rate... |
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| Keywords: | Sugarcane Shoot tips Embryogenesis Virus-free |
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