欧盟鱼病水平测试样品中DNA病毒检测 |
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引用本文: | 徐晔,段宏安,周毅,丁超. 欧盟鱼病水平测试样品中DNA病毒检测[J]. 安徽农业科学, 2012, 40(34): 16624-16626 |
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作者姓名: | 徐晔 段宏安 周毅 丁超 |
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作者单位: | 连云港出入境检验检疫局,江苏连云港,222042;连云港出入境检验检疫局,江苏连云港,222042;连云港出入境检验检疫局,江苏连云港,222042;连云港出入境检验检疫局,江苏连云港,222042 |
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基金项目: | 质检公益项目,国家质检总局科技计划项目,江苏检验检疫局科研项目 |
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摘 要: | [目的]通过参加欧盟鱼病水平测试,评价试验室检测能力,加强对检测方法的掌握。[方法]按照水平测试说明的方法进行病毒分离鉴定,测试的10个样品在病毒分离后用PCR方法检测DNA病毒。[结果]PTⅡ检测为流行性造血器官坏死病毒(Epizootic haemato-poietic necrosis virus,EHNV),PTⅢ为欧洲鮰病毒(European catfish virus,ECV),PTⅧ和PTⅩ为锦鲤疱疹病毒(Koi herpesvirus disease vi-rus,KHDV),PTⅨ为流行性溃疡综合征(Epizootic ulcerative syndrome,EUS)。[结论]该实验室检出了2011年欧盟鱼病水平测试样品中的4种DNA病毒,与最后公布的测试结果完全一致。
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关 键 词: | 鱼病水平测试 DNA病毒 欧盟 |
Detection of DNA Virus in the Inter-laboratory Proficiency Test |
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Affiliation: | XU Ye et al(Lianyungang Entry-Exit Inspection and Quarantine Bureau,Lianyungang,Jiangsu 222042) |
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Abstract: | [Objective] By participating the European Union inter-laboratory proficiency test for fish diseases,the detectability of the laboratory was evaluated and the master of the detection method was improved.[Method] Ten proficiency test samples were detected by virus isolation and identification according to the instruction of the proficiency test.[Result] The PTⅡ was epizootic haematopoietic necrosis virus(EHNV),the PTⅢ was European catfish virus(ECV),the PTⅧ and PTⅩ were both koi herpesvirus disease virus(KHDV),the PTⅨ was epizootic ulcerative syndrome(EUS).[Conclusion] Four types of DNA virus were detected positive from the proficiency test samples,which was consistent with the final results. |
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Keywords: | Proficiency test for fish disease DNA virus European |
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