杜仲叶提取物对人结直肠癌细胞HCT116及抗血管生成活性的影响

研究了杜仲叶提取物(EULE)对人结直肠癌细胞HCT116体外增殖和凋亡的影响以及其抗血管生成的活性。采用噻唑蓝(MTT)比色法测定了EULE及阳性对照药顺铂对HCT116和人脐静脉内皮细胞(HUVEC)体外增殖的影响;用流式细胞仪测定了EULE对HCT116细胞凋亡的影响。结果表明:EULE对HCT116细胞增殖具有一定的抑制作用,并表现出较好的量效关系,但抑制率稍低于顺铂;EULE对HCT116细胞的凋亡率的影响不显著;EULE对HUVEC细胞增殖的作用具有浓度依赖性,表现为低浓度促进增殖,高浓度抑制增殖。当EULE质量浓度分别为100、200、400和800 mg/L时,对HCT116细胞的抑制率分别为6.50%、10.52%、16.99%和56.49%,对HCT116细胞的凋亡率分别为0.00%、5.90%、7.00%和4.50%。EULE 800 mg/L时对HUVEC细胞的抑制率为41.59%,说明杜仲叶提取物只有达到一定浓度才具有抗新生血管生成的活性。

Effect of Eucommia ulmoides Oliv.Leaf Extracts on Human Colorectal Cancer Cells and Anti-angiogenesis Activities

The influences of Eucommia ulmoides Oliv.leaf extracts ( EULE) on proliferation and apoptosis of human colorectal cancer cell HCT116 in vitro, as well as the anti-angiogenesis activity of EULE , were investigated .MTT method was used to examine the effect of EULE and positive control drug-cisplatin on proliferation of HCT 116 and human umbilical vein endothelial cells ( HUVEC ) in vitro.Otherwise , flow cytometry instrument was used to determine the effect of EULE on apoptosis of HCT116.The results showed that EULE had a certain inhibition on HCT 116 cell proliferation, as well as the good dose-effect relationship, but the inhibition rate was slightly lower than the rate of inhibition of cisplatin .The impact of EULE on the rate of apoptosis of HCT116 cells was not obvious .The effect of EULE on HUVEC cell proliferation had a concentration-dependent manner, and it showed promoting effect at low concentrations and inhibiting effect at high concentration .The inhibition rates of 100, 200, 400, 800 mg/L EULE treatment group to HCT116 cell were 6.50%, 10.52%, 16.99%, 56.49%, and the apoptosis rates were 0.00%, 5.90%, 7.00%, 4.50%, respectively.The inhibition rate to HUVEC was 41.59% when the contrention of EULE was 800mg/L.This shows that the E.ulmoides Oliv.leaf extracts has anti-angiogenesis activity only when it reaches a certain concentration .